氩氦靶向治疗诱导A549人腺癌细胞凋亡及其相关基因的研究  

Apoptosis induced by argon-helium-based CRYOcare System in human A549 cells and the expression of related genes

作　　者：邹曰坤[1] 温居一[2] 冯华松[2] 段蕴铀[2] 

机构地区：[1]第二军医大学研究生院海军临床医学院,北京100037 [2]海军总医院呼吸内科,北京100037

出　　处：《现代肿瘤医学》2007年第8期1055-1057,共3页Journal of Modern Oncology

基　　金：全军"十一.五"医药卫生科研项目(06MB021

摘　　要：目的:探讨氩氦刀术后肺癌细胞凋亡及其可能机制。方法:A549细胞爬片后行氩氦刀冷冻,末端标记法(TUNEL)原位检测细胞凋亡,DNA电泳观察梯带,免疫组织化学SABC法检测bc1-2和bax表达。结果:在冷冻周边区,光镜形态学检查及TUNEL染色可见细胞凋亡,DNA电泳可见有梯带出现。免疫组化显示,bcl-2蛋白表达在冷冻前后无明显变化,bax蛋白在冷冻后其表达上调。结论:氩氦刀可以引起冷冻区周边部分细胞发生凋亡,其凋亡机制有bax蛋白参与,而与bcl-2无关。为增加冷冻疗效,术后可结合放化疗。Objective：To investigate the apoptosis and its possible mechanism of human A549 cells induced by argon -helium -based CRYOcare System in vitro. Methods：The A549 cells cultured on the cover glass were subject to cryo - treatment via CRYOcare Cryosurgical Unit. Then cell apoptosis was examined with terminal deoxynucleotidyl transferase -mediated dUTP nick end labeling（TUNEL） and DNA gel electrophores. The expression of bcl -2 protein and bax protein was detected by immunohistochemical procedures. Results： In the periphery of the cryolesion, apopotic cells can be determined by morphological analysis,TUNEL staining and DNA ladder. The expression of bax protein increased significantly,meanwhile the expression of bcl - 2 protein had no obvious change. Conclusion： The argon - helium - based CRYOcare System can induce cell apoptosis in the periphery of the cryolesion by upregulation of bax expression but not bcl - 2 expression. And chemotherapy or radiotherapy can be combined to improve the cryosurgery efficiency.

关 键 词：氩氦刀 冷冻 凋亡 

分 类 号：R730.56[医药卫生—肿瘤]