外阴鳞癌动物模型的建立及MDM2表达的研究  

Establishment of an animal model of vulvar squamous cell carcinomas and the expression of MDM2

作　　者：欧阳玲[1] 张淑兰[1] 付凌捷[1] 王欣[1] 陈荣莉[1] 

机构地区：[1]中国医科大学附属二院妇产科,辽宁沈阳110004

出　　处：《现代肿瘤医学》2007年第8期1062-1065,共4页Journal of Modern Oncology

基　　金：辽宁省自然科学基金资助项目(项目编号:20042067);辽宁省教育厅攻关计划项目(项目编号:2004D178)

摘　　要：目的:探讨外阴鳞癌动物模型建立的方法,研究在鼠外阴癌变发生、演进过程中MDM2的表达变化。方法:采用苯甲酸雌二醇注入32只129/J型鼠腹部皮下,8只为对照组,观测肿瘤的体积,取材做病理诊断。采用RT-PCR技术检测MDM2基因的mRNA表达。结果:外阴上皮内瘤变(VIN)发生率为65.5%(19/29),其中VIN1、VIN2各5例,VIN39例;外阴鳞状细胞癌(VSCC)发生率为34.5%。VIN1～2、VIN3、VSCC和对照组中,MDM2基因的mRNA的相对表达量分别为0.34±0.11、0.42±0.20、1.84±0.32和0.30±0.01;VSCC分别与VIN1～2、VIN3和对照组相比,差异均有统计学意义(P<0.05)。结论:129/J型鼠可为外阴病变(VINs/VSCC)的研究提供动物模型。MDM2在鼠外阴鳞癌中的变化与人组织相同,MDM2可作为外阴癌变的生物学指标。Objective：To study the way of establishing an animal model for the development of vulvar squamous cell carcinomas and analyze the expression of MDM2 mRNA. Methods： Estradiol benzoate hypodermically was injected to the abdomen of 32 129/J mice on the dose of 15mg/kg, no treatment to control group. Samples were observed under the light microscope. The expression of MDM2 mRNA was detected with RT - PCR. Results： VIN of mice was found in slice that stained by HE method, the incidence of VIN was 65.5%, there were 5 cases of VIN1 , 5 cases of VIN2, and 9 cases of VIN3. The incidence of VSCC was 34.5%. MDM2 mRNA was highly expressed in the VIN1-2, VIN3 and VSCC tissue, the expression of MDM2 had not been found in the vulvar epithelium of the comparison mice, there was no statistical significance between the comparison group and VINs group （P 〉 0.05）. there was statistical significance between comparison group and VSCC group or between VINs group and VSCC group （ P 〈 0.05 ）. Conclusion： Formation a VINs/VSCC model of mice successfully by using artificial estrogen. The change of MDM2 in VSCC of mice is the same as which in human tissue, so we can use MDM2 as a biological marker of VSCC.

关 键 词：外阴鳞癌动物模型 MDM2 129/J型鼠 

分 类 号：R734.2[医药卫生—肿瘤]