黑色素瘤抗原基因MAGE-1、MAGE-3和抑癌基因p53在肺癌中表达的研究  

Expression of MAGE-1,MAGE-3 and p53 genes in lung carcinomas

作　　者：李秋泽[1] 董子明[2] 赵国强[2] 彭培立[1] 赵洁[1] 

机构地区：[1]解放军第155中心医院,河南开封475003 [2]郑州大学基础医学院,河南郑州450052

出　　处：《现代肿瘤医学》2007年第8期1106-1108,共3页Journal of Modern Oncology

摘　　要：目的:分析肺癌组织MAGE-1和MAGE-3基因以及p53基因表达及其相互关系。方法:取肺癌组织标本30例,另取癌周组织作为对照。采用逆转录聚合酶链反应(RT-PCR)方法,检测MAGE-1和MAGE-3基因以及p53基因的表达。结果:MAGE-1,MAGE-3在肺癌中有较高表达,MAGE-3的表达高于MAGE-1的表达。p53在肺癌中表达较低,在正常组织中表达较高;MAGE-1在肺腺癌中表达较鳞癌高,MAGE-3在肺鳞癌中表达较高;MAGE-1,MAGE-3的表达可能与肿瘤分化程度无关;p53在小细胞肺癌中的表达有待进一步研究;p53与MAGE在体内表达成负相关。结论:MAGE-1、MAGE-3和p53在肺癌组织与正常组织中有不同的表达,MAGE-1、MAGE-3的表达与病理类型有关,与肿瘤分化无关。Objective：In this study, we analyzed the expression of MAGE - 1, MAGE -3 and p53 genes in lung cancers and assessed the relationship among them. Methods： Using polymerase chain reaction（ RT - PCR） methods, we studied 60 specimen that were obtained from surgical samples or fiber bronchoscope（FB） biopsy including 30 lung carcinoma and 30 matched adjacent normal tissues. Results： MAGE - 1 and MAGE -3 genes showed high expression in lung cancer . The expression of MAGE - 3 gene was higher than that of MAGE - 1 gene in lung cancer, p53 gene expression is low in lung carcinomas but high in normal tissues ;The expression of MAGE - 1 gene was higher in adenocarcinomas than that in squamous cell carcinomas. Otherwise the expression of MAGE -3 gene was higher in squamous cell carcinomas than that in adenocarcinomas ;There is no direct relation between the tumor differentiation degree and the expression of MAGE - 1, MAGE - 3 genes. The expression of p53 possibly needs more studies in small cell lung cancer;The expression between p53 gene and MAGE genes probably had inverse correlation. Conclusion： The expression lecels of MAGE - 1, MAGE - 3 and p53 genes are different in lung carcinoma and normal tissues . The expression of MAGE - 1, MAGE - 3 are related to the pathological and not related to the tumor differentiation degree.

关 键 词：肺癌 MAGE-1 MAGE-3 p53 逆转录聚合酶链反应 

分 类 号：R734.2[医药卫生—肿瘤]