β-甘露聚糖酶降解预胶化淀粉的条件研究  

Effects of Optimized Conditions on β-Mannanase Degradating Pregelatinized Starch

作　　者：李志东[1] 李娜[1] 邱峰[1] 张洪林[1] 李长波[1] 赵国峥[1] 

机构地区：[1]辽宁石油化工大学环境工程系,抚顺113001

出　　处：《精细石油化工进展》2007年第1期47-50,共4页Advances in Fine Petrochemicals

基　　金：辽宁省自然科技基金项目99105005。

摘　　要：实验考察了影响β-甘露聚糖酶降解预胶化淀粉因素:缓冲液pH值、反应时间、底物用量、反应温度及酶用量。确定了降解的最佳条件条件:缓冲液pH7,反应时间2h,预胶化淀粉浓度5mg/mL,温度90℃,酶加入量5mL。设计一个L16(45)正交实验,确定加热温度为反应过程中的显著因素。In this experiment, the factors, including pH, reaction time, concentration of substance, reaction temperature and enzymes amounts, which can influence β - mannanase degradating pregelatinized starch, were studied. The optimum conditions for best degradation were as follows ： ptI of reaction system buffer solution was 7, reaction time 2 hours, concentration of substance 5 mg/mL, reaction tempratue 90℃, enzymes amounts 5 mL. An orthorgonal test of L16 （45 ） was designed, which showed heating temprature was prominent factor.

关 键 词：预胶化淀粉 Β-甘露聚糖酶 酶解条件 

分 类 号：TS231[轻工技术与工程—粮食、油脂及植物蛋白工程]