铜针留置兔耳缘静脉血铜变化和对肝脏组织及生化指标影响的研究  

CHANGES IN SERUM Cu^(2+) AND INDEXES FOR LIVER PATHOLOGY AND BIOCHEMISTRY BEFORE AND AFTER COPPER NEEDLE RETAINED IN CENTRAL VEIN OF RABBIT EARS

作　　者：杨晓楠[1] 殷国前[1] 郑承杰[2] 唐小岚[2] 李伟雄[1] 罗莉[3] 苏承武[1] 付时章[4] 

机构地区：[1]广西医科大学第一附属医院整形美容科 [2]广西壮族自治区卫生厅 [3]广西医科大学附属口腔医院 [4]江西省妇幼保健院

出　　处：《中国修复重建外科杂志》2007年第8期837-841,共5页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：广西壮族自治区卫生厅重点科研课题基金资助项目(200504)~~

摘　　要：目的探讨铜针留置前后血清铜浓度变化和释放的铜离子对体内肝脏组织及生化指标所产生的影响。方法将49只新西兰兔随机分成5组,A组留置铜针1d、B组留置铜针3d、C组留置铜针5d、D组留置铜针7d,每个实验组10只,E组(留针前,正常对照组)9只。分别于留针前及留针后1、3、5和7d,抽静脉血检测血清铜离子浓度、肝功能指标,同期切取部分肝脏行HE染色,对出现阳性结果的组织标本行组织化学特殊染色、透射电镜检查。结果铜针留置后,铜离子浓度随时间的延长而增加。A、B、C、D组分别为1.40±0.49、1.45±0.53、2.01±0.40、2.38±0.83μg/ml,D组与E组(1.34±0.45μg/ml)比较有统计学意义(P<0.05);肝功能ALT与AST指标变化各组间比较无统计学意义(P>0.05),但在1、5及7d表现出较强的正相关关系,相关系数分别为0.686(P<0.05)、0.712(P<0.05)和0.768(P<0.01)。组织学检查,肝脏组织可见无菌性炎性反应,并随时间延长逐渐消退,Masson三色染色及Ag染色无明显肝小叶结构改变及肝组织纤维化。结论铜针留置兔耳缘静脉后对肝脏未产生明显的毒副作用。Objective To explore the changes in the serum Cu^2＋ and the indexes for the liver pathology and biochemistry before and after the copper needle retained in the central veins of the rabbit ears. Methods Forty-nine New Zealand rabbits were randomly divided into 5 groups： Group A （copper needles retained for 1 d）, Group B （3 d）, Group C （5 d）, Group D （7 d）, and Group E （the control group, without any copper needles retained）. In each experimental group, there were 10 rabbits, and in the control group there were 9 rabbits. The rabbits in each group were arranged to have their venous blood drawn for determination of the Cu^2＋ concentration, and for observation on the changes in the liver biochemical indexes for 5 times before and after the copper needles were retained in the central veins of the rabbit ears. At the same time, a piece of the liver tissue in each rabbit was taken for examination of the pathological changes. All the liver samples were given the basic pathological examination; if the liver sample had some extraordinary pathological features, the specific pathological examination would be given, even using the transmission electron microscope. Results After the copper needles were retained in the central veins of the rabbit ears, the Cu^2＋ concentration increased with the passing time. The concentrations in the groups were 1.40±0. 49 μg/ml in Group A, 1.45±0.53μg/ml in Group B, 2.01±0.40 μg/ml in Group C, 2.38±0.83 μg/ml in Group D, and 1.34±0. 45 μg/ml in Group E, respectively. There was a statistically significant difference between Group D and Group E （P〈 0. 05）. There were no significant changes in the ALT and AST levels when compared with those before the copper needles were retained（P〉0. 05）; however, there was a considerably positive correlation on 1st day （r=0. 686, P〈0. 05）, 5th day （r=0.712, P〈0.05）, and 7th day （r=0.768, P〈0.01） when compared with those after the copper needles were retained. The histological examination showed

关 键 词：铜针 铜离子 肝功能 病理学 血管瘤 

分 类 号：R732.2[医药卫生—肿瘤]