内生枯草芽孢杆菌B-001菌株内生定殖研究及生物学特性  

ENDOPHYTE B-001(Bacillus subtilis) COLONIZATION AND ITS BIOLOGICAL CHARACTERS

作　　者：易有金[1] 罗坤[2] 罗宽[1] 刘二明[1] 

机构地区：[1]湖南农业大学生物安全科技学院,湖南长沙410128 [2]广西大学农学院,广西南宁530005

出　　处：《核农学报》2007年第4期349-352,共4页Journal of Nuclear Agricultural Sciences

基　　金：湖南省教育厅重点资助项目(02A019)

摘　　要：用浸种、浸根、淋根、伤茎、伤叶和喷雾等接种方法测试枯草芽孢杆菌B-001菌株入侵烟草植株的途径,发现该菌可通过自然孔口和伤口入侵寄主植物;取接种植株的茎部组织切片在电镜下观察,发现该菌株在烟草的微管束和细胞内定殖。生长特性研究结果表明该菌株最适生长pH为7.5,最适生长温度为30℃,48～96h内处于稳定生长期。The different inoculation methods,such as dipping seed,dipping root,watering root,injecting stem,injecting leaf,praying were used to test how the endophyte B-001（Bacillus subtilis） entered the host plant.The results indicated that the bacteria penetrated into plant tissues through natural wounds.Observing the plant tissue after inoculation 30 days by electron microscope,strain B001 could be found in vascular and cell.Study on its biological character showed that the optimal pH and temperature for the growth of B-001 were 7.5 and 30℃,respectively,and its stable growth stage was 48～96h.

关 键 词：烟草 内生枯草芽孢杆菌B-001 内生定殖 生物学特性 

分 类 号：S476[农业科学—农业昆虫与害虫防治]