TNF-α基因转染对胃癌细胞系的生长抑制效应  

Suppression of Gastric Cancer Cell Line Growth After Transfection of TNF-a Gene

作　　者：邹卫[1] 王丽君[1] 张乃蘅 

机构地区：[1]北京医科大学生化与分子生物学系,北京100083

出　　处：《中国肿瘤生物治疗杂志》1997年第1期47-50,共4页Chinese Journal of Cancer Biotherapy

摘　　要：本工作以逆转录病毒为载体将TNF-α基因导入胃癌细胞系MKN28和BGC823,经筛选获阳性克隆MKN28-TNF和BCC823-TNF.对TNF基因在肿瘤细胞中的表达及其对肿瘤细胞的作用进行了探讨.证明外源TNF基因在肿瘤细胞中获得表达;肿瘤细胞生长速度明显减慢,生长抑制率分别达71%和 66%.HTdR掺入率在TNF基因转染细胞明显降低.转染后的肿瘤细胞丧失了裸鼠体内成瘤能力.实验结果表明TNF基因导入胃癌细胞后,细胞生长及致癌能力受到明显抑制,为TNF最终能用于临床胃癌基因治疗提供了有意义的资料.Human gastric cancer cell line MKN28 and BGC823 were infected with recombinated retroviruses containing TNF-α gene, The expression and function of TNF were identified in transfected cancer cell line MKN28-TNF and BGC823-TNF. Cancer cells showed lower growth rate after transfection, and the inhibition of growth rate were 71% and 66% in MKN28-TNF and BGC823-TNF in comparison with the control, respectively. Decreased levels of ~3 HTdR incorporation were observed. The tumorigenicity of MKN28-TNF in nude mice was completely lost as compared with the control . The results demonstrate the inhibition of gastric cancer cells growth and tumorigenicity introduced by exogenous TNF gene.

关 键 词：胃肿瘤 肿瘤坏死因子 基因疗法 

分 类 号：R735.205[医药卫生—肿瘤]