PDCD5在成人急性髓性白血病骨髓细胞的异常表达  

Abnormal Expression of PDCD5 in the Bone Marrow Cells of Adult Acute Myeloid Leukemia

作　　者：阮国瑞[1] 陈珊珊[1] 马曦[2] 常艳[1] 王卉[1] 付家瑜[1] 秦亚溱[1] 李金兰[1] 刘艳荣[1] 

机构地区：[1]北京大学人民医院,血液病研究所,北京100044 [2]北京大学疾病基因研究中心,北京100083

出　　处：《中国实验血液学杂志》2007年第3期462-465,共4页Journal of Experimental Hematology

基　　金：国家自然科学基金(编号30370590);国家"211工程"北京大学人类功能基因和疾病基因学科群基金资助项目(编号215)

摘　　要：为了研究成人急性髓性白血病(acute myeloid leukemia,AML)骨髓细胞凋亡促进分子---PDCD5的表达,以探讨PDCD5在AML发病机制中的作用,利用21种不同荧光标记的单克隆抗体标记骨髓细胞,通过流式细胞术检测AML病人及正常人骨髓不同群细胞的胞内PDCD5的表达。部分标本进行蛋白印迹实验检测AML病人及正常人骨髓细胞内PDCD5的表达。结果表明:36例未治AML病人骨髓总的有核细胞内PDCD5平均荧光强度明显低于30例正常人组,分别为3059±1392:7432±1261(P<0.01),其中未治AML病人骨髓粒细胞、单核细胞、幼稚细胞及淋巴细胞内PDCD5平均荧光强度均低于相应的正常人组骨髓细胞,分别为3939±2121:8367±1045;3156±1635:5917±2329;2824±1592:3998±2106;1474±816:3355±2042(P值均小于0.01)。部分标本进行蛋白印迹检测的结果也显示AML病人骨髓细胞内PDCD5的表达低于正常人。结论:未治AML病人骨髓总有核细胞的PDCD5表达低于正常人,未治AML病人骨髓粒细胞、单核细胞、幼稚细胞及淋巴细胞内PDCD5表达均低于正常人组骨髓细胞。PDCD5的异常表达可能在AML的病理机制中起到一定的作用。The objective of this study was to estimate a novel apoptosis-promoting molecule PDCD5 expression in the bone marrow cells from adult acute myeloid leukemia （AML） for investigation of its significance in the pathogenesis of AML. Flow cytometry assay was used for detection of PDCD5 expression in the different groups of cells from bone marrow of AML patients and normal controls by using 21 monoclonal antibodies with different fluorescent markers. The PDCD5 expressions in bone marrow cells from some AML patients and normal controls were also detected by Western blot. The results showed that the mean PDCD5 fluorescence intensity in bone marrow nucleated cells （ MNC ） from the bone marrow of 36 untreated AML patients was significantly lower than that from the bone marrow of 30 normal controls （ 3059 ± 1392） vs （7432 ± 1261 ） （ P 〈0.01 ）. The mean PDCD5 fluorescence intensity was lower in the marrow granulocytes, monocytes, blast cells, and lymphocytes from untreated AML patients than that from normal （3939 ± 2121 ） vs （8367 ± 1045） ; （3156 ± 1635） vs （5917±2329） ; （2824± 1592） vs （3998±2106） ; （ 1474 ±816） vs （3355±2042） respectively, （all P 〈 0.01 ）. Western blot analysis demonstrated that PDCD5 expression was significantly decreased in the AML cells, as compared with normal cells. It is concluded that PDCD5 expression in MNC in untreated AML patients is lower than that in the normal. PDCD5 expression in the marrow granulocytes, monocytes, blast cells, and lymphocytes of untreated AML patients is significantly lower than that in the normal. It suggests that the abnormally low expression of PDCD5 may be involved in the pathogenesis of AML.

关 键 词：急性髓性白血病 PDCD5 骨髓细胞 细胞凋亡 

分 类 号：R733.71[医药卫生—肿瘤]