苦参碱诱导多发性骨髓瘤细胞株RPMI8226凋亡的实验研究  

Apoptosis of Multiple Myeloma RPMI8226 Cell Line Induced by Matrine

作　　者：杨军军[1] 高申孟[2] 陈慧[1] 

机构地区：[1]浙江温州医学院附属第二医院血液学室,温州325027 [2]温州医学院附属第一医院医学研究所,温州325000

出　　处：《中国实验血液学杂志》2007年第3期515-518,共4页Journal of Experimental Hematology

摘　　要：本研究探讨苦参碱对人骨髓瘤细胞株RPMI8226的作用及其机制。用不同浓度苦参碱处理RPMI8226细胞24、48小时,通过形态学观察、Annexin-V分析、DNA琼脂糖电泳、线粒体膜电位检测观察苦参碱对RPMI8226细胞的促凋亡作用。结果表明:RPMI8226细胞经苦参碱处理后,出现典型的细胞凋亡形态,凋亡早期细胞膜外翻,DNA琼脂糖电泳出现典型的梯状结构,线粒体膜电位崩塌。结论:苦参碱能有效地诱导骨髓瘤细胞株RPMI8226细胞凋亡,凋亡率与药物剂量和作用时间呈依赖性。This study was aimed to investigate the effect of matrine on multiple myeloma RPMI8226 cell line and its mechanism. The RPMI8226 cells were treated with various concentrations of matrine for 24 and 48 hours ; the promotingapoptosis effect of matrine on RPMI8226 cells was observed by morphology, Annexin-V analysis, DNA agarose gel electrophoresis, mitochondrial transmembrane potential detection, etc, The results indicated that after treatment of RPMI8226 cells with matrine, the typical morphology of cell apoptosis appeared, Annexin-V analysis showed positive, the typical ladder was observed in DNA agarose gel electrophoresis, and the mitochondrial transmembrane potential decreased. It is concluded that the matrine can effectively inhibite the RPM8226 cell growth and reduce cell viability in dose-and time-dependent manner, It is presumed that the matrine induces RPMI8226 cell apoptosis through mitochondrial signaling pathway.

关 键 词：苦参碱 多发性骨髓瘤 RPMI8226细胞株 细胞凋亡 

分 类 号：R733.3[医药卫生—肿瘤] R979.1[医药卫生—临床医学]