T细胞急性淋巴细胞白血病免疫表型分析  

Immunophenotyping Characteristics of T-cell Acute Lymphoblastic Leukemia

作　　者：陈丽娟[1] 李建勇[1] 吴雨洁[1] 杨慧[1] 钱思轩[1] 吴汉新[1] 陆化[1] 徐卫[1] 盛瑞兰[1] 

机构地区：[1]南京医科大学第一附属医院,江苏省人民医院血液科,南京210029

出　　处：《中国实验血液学杂志》2007年第4期692-695,共4页Journal of Experimental Hematology

基　　金：江苏省135工程医学重点人才资助项目(编号RC2002044);江苏省人民医院人才启动基金项目(编号155NA0505)

摘　　要：本研究探讨T细胞急性淋巴细胞白血病(T-ALL)免疫表型特点。应用一组系列相关单克隆抗体和CD45/SSC设门的三色流式细胞术对140例T-ALL进行免疫表型检测。结果显示,白血病细胞表面T系抗原表达依次为CD7>CD2>CD3>CD5,近20%患者CD10表达阳性。140例患者中12例带有B系抗原标记,阳性率达8.57%;136例中31例带有髓系抗原标记,阳性率达22.79%,所有病例均无CD14表达。干/祖细胞标记CD34表达阳性率31.06%,41例CD34+T-ALL髓系抗原表达15例,阳性率36.59%,91例CD34-T-ALL髓系抗原表达14例,阳性率15.38%,二者具有统计学差异(p<0.01)。儿童T-ALLCD3表达高于成人(p<0.05),而CD33表达明显低于成人(p<0.01)结论:免疫表型分析是诊断T-ALL的重要手段,T-ALL免疫表型具有异质性。The objective of this study was to investigate the immunophenotypic characteristics of T-cell acute lymphoblastic leukemia （T-ALL）. Immunophenotyping was performed in 140 T-ALL patients by flow cytometry using a panel of monoclonal antibodies and CD45/SSC gating. The results showed that the T-lineage-associated antigen expressions were CD7 〉 CD2 〉 CD3 〉 CD5 successively. The positive rate of CD10 was 19.42% in patients. Among 140 cases of T-ALL, 12 （8.57%） was accompanied by B-lineage associated antigen expression. Myeloid antigen expression was identified in 31 out of 136 cases （22.79%）. None of them expressed CD14 antigen. The positive rate of CD34 was 31.06%. The positive rate of myeloid antigen expression in CD34^＋ T-ALL （36.58 % ） was significantly higher than that in CD34^- T-ALL （ 15.38% ） （p 〈 0.01 ）. The expression of CD3 in child T-ALL was higher than that in adult T-ALL, whereas the expression of CD33 in children was lower than that in adults. It is concluded that immunophenotyping is an important tool for diagnosis of T-ALL. Immunophenotypic characteristics of T-ALL is heterogeneous.

关 键 词：T细胞急性淋巴细胞白血病 急性白血病 免疫表型 

分 类 号：R733.71[医药卫生—肿瘤]