凋亡相关基因pnas-2的表达与白血病关系  

Correlation between Expression of Apoptosis-Related Gene pnas-2 and Leukemia

作　　者：黄洪晖[1] 朱坚轶[1] 钟济华[1] 王海嵘[1] 钟华[1] 韩洁英[1] 陈芳源[1] 

机构地区：[1]上海交通大学医学院附属仁济医院血液科,上海200001

出　　处：《中国实验血液学杂志》2007年第4期738-742,共5页Journal of Experimental Hematology

基　　金：国家自然科学基金资助项目;编号30271660

摘　　要：本研究旨在探讨凋亡相关基因pnas-2与白血病的关系。应用RT-PCR技术检测硫化砷作用前后NB4、K562、U937细胞pnas-2基因的表达及急性白血病病人治疗前后pnas-2基因表达的变化。结果显示:NB4细胞在硫化砷作用后pnas-2表达有明显下调,并呈时间依赖性;而K562及U937细胞在硫化砷作用后pnas-2基因的表达无明显变化。急性白血病病人治疗前pnas-2基因表达阳性率为100%,显著高于正常对照组;经非硫化砷诱导化疗后,完全缓解病人pnas-2基因的表达全部转为阴性;而未达到缓解的病例pnas-2基因的表达仍为阳性,较化疗前无明显变化。结论:pnas-2基因可能与硫化砷诱导APL细胞凋亡密切相关,同时其表达有可能作为急性白血病分子生物学缓解的指标之一。The study was purposed to explore the correlation between apoptosis-related gene pnas-2 and leukemia. The RT-PCR was performed to detect the expression levels of pnaso2 gene in NB4, K562, U937 cells before and after treatment with AS4 S4 , and to analysis the expression change of pnas-2 gene in bone marrow cells from patients with acute leukemia before and after chemotherapy. The results showed that the expression of pnas-2 gene in arsenic sulfide treated NB4 cells was down regulated in time-dependent manner, but the same outcome in K562 and U937 cells after being treated with AS4S4 was not found. The positive expression rate of pnas-2 in cells from untreated patients with acute leukemia was 100%, and was significantly higher than that in normal control group. After chemotherapy, the expression was negative in complete remission patients, whereas in no-remission patients there were no significant differences of expression of pnas-2 before and after treatment. It is concluded that the pnas-2 gene may be closely related with apotosis of arsenic sulfide treated APL cells, and may consider as a molecular biological remission marker in acute leukemia.

关 键 词：pnas-2基因 硫化砷 白血病 

分 类 号：R733.7[医药卫生—肿瘤]