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作 者:吴佩[1] 茆家定[1] 梁林虎[1] 闫竞一[1]
机构地区:[1]皖南医学院弋矶山医院普外科,安徽省芜湖市241001
出 处:《世界华人消化杂志》2007年第17期1921-1927,共7页World Chinese Journal of Digestology
基 金:安徽省自然科学基金;No 03043740;安徽省教育厅自然科学基金;No 2006Kj115c
摘 要:目的:探讨大肠癌组织中胃泌素(GAS)、生长抑素(SS)的表达及其比势与细胞周期调控蛋白P16^(INK4a)、P21^(CIP1)及细胞周期素(Cyclins)、细胞周期依赖性蛋白激酶(CDKs)表达的关系.方法:随机选择79例大肠癌患者的手术切除标本,采用免疫组化SP法检测GAS、SS、P16^(INK4a)、P21^(CIP1)、Cvclin D1、Cvclin E、Cyclin A、Cyclin B1、CDK2、CDK4的表达情况.结果:Cyclin D1、CDK2、CDK4、Cyclin A在GAS高表达组、中表达组的阳性表达率明显高于低表达组;而P16^(INK4a)、P21^(CIP1)阳性表达率与此相反.cyclin E在SS低表达组的阳性表达率明显高于中表达组、高表达组;P21^(CIP1)在ss高表达组、中表达组的阳性表达率明显高于低表达组;CDK2在SS低表达组的阳性表达率明显高于SS高表达组.大肠癌组织中GAS、SS表达的积分比值(GAS/SS)与Cyclin D1(r =0.252)、Cyclin E(r=0.387)、Cyclin A(r= 0.466)、CDK2(r=0.519)、CDK4(r=0.434)呈正相关(P<0.01)与P16^(INK4a)(r=-0.385)、P21^(CIP1)(r =-0.454)蛋白的表达积分呈负相关(P<0.01).结论:GAS、SS对大肠癌细胞生长的调控可能与P16^(INK4a)、P21^(CIP1)、Cvclin D1、Cvclin A、CDK2、CDK4、cvclin E基因异常表达有关.GAS对大肠癌细胞周期的调控位点可能在G1、S、G2期,SS对大肠癌细胞周期的调控位点可能在G1/S、G2/M期的交界点,即S、M期的入口.对大肠癌GAS/SS积分比值分析、可作为临床大肠癌生物学行为的重要评估指标.AIM: To explore correlations among the expression of gastrin (GAS), somatostatin (SS), P16^INK4a, P21^CIP1, Cyclins, and Cyclin-dependentkinases (CDKs) in large intestine carcinomas. METHODS: Seventy-nine resected large intestine carcinomatous specimens were randomly selected. GAS, SS, P16^INK4a, p21^CIP1, Cyclin D1, Cyclin E, Cyclin A, Cyclin B1, CDK2, and CDK4 were de- tected by immunohistochemistry (Streptavidin- Peroxidase, SP). RESULTS: Positive expression rates of Cyclin D1, CDK2, CDK4, and Cyclin A were significantly higher in cases showing high or medium expression levels of GAS than in cases with low GAS expression. The positive expression rates of P16^INK4a and P21^CIP1 were the reverse. The positive expression rate of Cyclin E was significantly lower in the high and middle SS expression groups than in the low SS expression group. The positive expression rate of P21^CIP1 was significantly higher, and that of CDK2 was significantly lower, in the high and middle SS expression groups compared with the low SS expression group. The integral GAS/SS was positively correlated with Cyclin D1 (r = 0.252), Cyclin E (r = 0.387), Cyclin A (r = 0.466), CDK2 (r = 0.519), and CDK4 (r = 0.434, P 〈 0.01), but negatively correlated with P16n^K~ (r = -0.385) and P21cnn (r = -0.454, P 〈 0.01). CONCLUSION: The regulation and control of GAS and SS in large intestine carcinoma cell growth may be directly related to the abnormal expression of P16^INK4a, P21^CIP1, Cyclin D1, Cyclin A, CDK2 CDK4, and Cyclin E. The integral GAS/SS may be considered an important evaluating target for clinical determinations of the biological behavior of large intestine carcinomas.
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