用原位 PCR 研究石蜡包埋肝组织中微量 HCV RNA  

DETECTION ON HCV RNA IN PARAFFIN EMBEDDED LIVER TISSUES BY IN SITU POLYMERASE CHAIN REACTION

作　　者：周元平[1,2] 彭文伟[1,2] 姚集鲁 张志刚[1,2] 罗光汉 邓子德[1,2] 顾秀华 

机构地区：[1]中山医科大学附属第三医院传染病科 [2]广西医科大学附属第一医院传染病科

出　　处：《中山医科大学学报》1997年第2期100-103,共4页Academic Journal of Sun Yat-sen University of Medical Sciences

基　　金：国家教委博士点基金;CMB资助

摘　　要：为定位分析石蜡包埋肝组织中微量ＨＣＶＲＮＡ，改良建立了原位ＰＣＲ检测技术，并对１７例丙型肝炎相关的肝细胞癌患者癌组织和癌周肝组织石蜡包埋切片进行检测。结果显示：该技术的特异性可靠，敏感性高于原位杂交法；两种组织切片ＨＣＶＲＮＡ阳性率分别为４１．２％和７０．６％，阳性信号主要呈胞浆型分布；在癌细胞中核型和核膜型分布比例较癌周肝组织明显增高，提示ＨＣＶ可能与宿主细胞基因存在着相互作用。In order to localize a trace of HCV RNA in tissue sections from patients with hepatocellular carcinoma (HCC), a technique of in situ polymerase chain reaction was developed and carried out in paraffin embedded sections of cancerous and pericancerous liver tissues from 17 HCC patients. The results showed that the specificity of the technique was satisfactory. The positive rates of HCV RNA in sections of cancerous and pericancerous liver tissues were 41.2% and 70.6% respectively. The positive signals mainly localized in cytoplasm. But in cancerous tissues, the positive signals in nuclei and nuclear membrane were significantly increased. These results further support the association between HCC and HCV infection and suggest the interaction between HCV gene and host genome.

关 键 词：聚合酶链反应 丙型肝炎病毒 肝肿瘤 遗传学 

分 类 号：R512.63[医药卫生—内科学] R735.703[医药卫生—临床医学]