抗癌药物诱导人膀胱癌 BIU-87 和 E-J 细胞凋亡的作用  

Chemotherapy and apoptosis of BIU and E J bladder cancer cells

作　　者：赵长林[1,2] 刘佳[1,2] 李宏 罗阳[1,2] 李光伟 李学孔[1,2] 冯世民[1,2] 曾庆有 江岩[1,2] 郭丽 

机构地区：[1]沈阳医学院附属中心医院泌尿外科 [2]沈阳医学院分子生物学研究室

出　　处：《中华泌尿外科杂志》1997年第5期286-288,共3页Chinese Journal of Urology

摘　　要：应用分子生物学方法，观察了丝裂霉素、喜树碱、顺铂和阿霉素对膀胱癌细胞系ＢＩＵ８７和ＥＪ体外生长和生存的影响，对抗癌药物诱导膀胱癌细胞凋亡作用进行了研究。结果：四种抗癌药均能在不同程度上抑制膀胱癌细胞生长，诱导膀胱癌细胞凋亡的能力与其生长抑制效应成正比。细胞形态学和ＤＮＡ分析显示喜树碱、顺铂处理的ＢＩＵ８７和丝裂霉素、阿霉素处理的ＥＪ细胞呈现典型的凋亡现象。揭示药物的抑癌作用是通过诱导肿瘤细胞凋亡实现的，它可能是抑制肿瘤生长的机理之一。不同膀胱癌细胞系对药物诱导凋亡的敏感性存在着差异。细胞凋亡现象为肿瘤化疗提供了新的领域。The effect of some chemotherapeutic agents on the cell growth of BIU and E J bladder cancer cell lines was investigated. The in vitro cell growth inhibition and the hallmarks of apoptosis of BIU cells treated with 10 hydroxycamptothecini and cisplatin and E J cells treated with mytomycin c and adriamycin were studied. Apoptosis of the cancer cells was directly related to the growth suppression by these agents. It was suggested that the death of BIU and E J cancer cells induced by these chemotherapeutic drugs was through the apoptosis pathway. Different cancer cells responded differently to the anticancer drugs. The induction of apoptosis might be a new field in the study of anticancer chemotherapy.

关 键 词：膀胱肿瘤 癌 细胞凋亡 BIU-87 E-J 抗癌药 

分 类 号：R737.140.5[医药卫生—肿瘤] R979.1[医药卫生—临床医学]