HP感染蒙古沙土鼠致Barrett食管及胃癌的实验研究  

Study of Barrett esophagus and gastric carcinoma in Mongolian gerbils induccd by Helicobacter pylori infection

作　　者：兰春慧[1] 房殿春[2] 邹全明[3] 

机构地区：[1]第三军医大学大坪医院消化内科,重庆400042 [2]第三军医大学西南医院消化内科,重庆400038 [3]第三军医大学检验系微生物及免疫学教研室,重庆400038

出　　处：《重庆医学》2007年第15期1491-1493,共3页Chongqing medicine

基　　金：国家自然科学基金资助项目(39870345);全军"十五"重点基金资助项目(01Z075)

摘　　要：目的通过建立幽门螺杆菌(HP)感染蒙古沙土鼠的动物模型,观察HP及HP与N-甲基-N′-硝基-N-亚甲基胍(MNNG)共同作用后食管和胃黏膜的组织学改变。方法96只SPF级蒙古沙土鼠随机分为4组,每组24只。A组单用HP菌液灌胃;B组在接种HP后4周,摄入MNNG水(20μg/ml),连续30周;C组单用MNNG水(20μg/ml),连续30周;D组为对照组。各组分别在实验后12、24和48周3个时相点各处死8只,取食管和胃黏膜行组织学检查,用Warthin-Starry银染、PCR和快速尿素酶法检测HP。结果累计至48周,萎缩、肠化生和异型增生的发生率A组(62.5%,33.3%,37.5%)、B组(62.5%,20.8%,54.2%)显著高于C组(37.5%,4.2%,8.3%)、D组(0),差异有统计学意义(P<0.01)。A组有1例发生胃癌,2例出现Barrett食管。结论HP感染可以直接导致胃癌发生,同时也能诱发出Barrett食管。Objective Using a model of Helicobacter pylori（HP） infected Mongolian gerbil,to observe the effect of HP and N- methy-N＇ nitro-N-nitrosoguanidine（MNNG） on gastric and esophagus mucosa. Methods A total of 96 Mongolian gerbils, sixweek-old, were randomly divided into four groups （each 24 animals） ：group A infected with HP group B infected with HP followed by MNNG administration group C received MNNG without HP infection group D as control. Eight animals from each group were killed at 12,24,48 weeks,and histopathological changes in their stomach and esophagus were examined for chronic gastritis, intestinal metaplasia,atypical hyperplasia ,adenoma and Barrett esophagus. Results The incidences of intestinal metaplasia and dysplasia in group A and B were significantly higher than those in the other groups （P〈0.01）. Conclusion HP could directly induce gastric carcinoma and Barrett esophagus.

关 键 词：幽门螺杆菌 胃肿瘤 N-甲基-N'-硝基-N-亚甲基胍 BARRETT食管 

分 类 号：R571[医药卫生—消化系统] R735.2[医药卫生—内科学]