姜油树脂/β-环糊精包合物的制备工艺优化和表征  

Preparation Optimization and Characterisation of Ginger Oleoresin/β-Cyclodextrin Inclusion Complex

作　　者：刘利萍[1] 王秀珍[1] 毛威 

机构地区：[1]绍兴文理学院化学化工学院药学系,浙江绍兴312000 [2]绍兴市药品检验所,浙江绍兴312000

出　　处：《中国药学杂志》2007年第15期1164-1167,共4页Chinese Pharmaceutical Journal

基　　金：浙江省教育厅资助项目(20041229);绍兴市科技局资助项目(2005162)

摘　　要：目的优化姜油树脂/β-CD包合物的制备工艺并对包合物进行表征。方法采用共沉淀法,以姜油树脂中姜酚的包合率为测定指标,通过均匀设计优化工艺参数;应用SEM,IR,DSC,TLC对包合物进行表征。结果姜酚的包合率与工艺参数的相关性为:Y=56.36-6.38X1+11.21X2+2.13X3-0.016X23(r2=0.981,P<0.05),在优化条件β-CD/姜油树脂质量比(X1)7/1、包合时间(X2)1h,包合温度(X3)65℃和的条件下,姜酚包合率达(95.20±0.31)%(n=3);SEM,IR,DSC图谱显示姜油树脂与β-CD形成了包合物,TLC图谱说明姜油树脂包合前后的基本组分没有改变。结论该制备工艺简单,优化工艺参数合理,控制工艺参数可以得到高包合率的产品。OBJECTIVE To optimize the preparation of ginger oleoresin/β-cyclodextrin inclusion complex and to characterize it. METHODS Inclusion complex was prepared by co-precipitation method and uniform design was applied to optimize the preparation parameters. The content of entrapped gingerol was determined by UV spectrophometry as the evaluating indicator of inclusion rate of ginger oleoresin. The inclusion complex was characterized by SEM, IR, DSC and TLC. RESULTS The statistic result indicated by SPSS10. 0 that the relationship between the inclusion rate and the preparation parameters was： Y = 56. 36 -6. 38X1 ＋ 11.21X2 ＋ 2. 13X3 -0. 016X3^2（r^2 =0. 981 ,P 〈0. 05）. The inclusion rate of gingerol was up to （95. 20±0. 31 ） % under the optimal conditions of mass ratio of β-CD/ginger oleoresin （X1） 7/1, and stirring time （X2 ） 1 h, inclusion temperature （X3 ） 65 ℃. The result of SEM, IR and DSC indicated that ginger oleoresin formed the inclusion complex withβ-CD. TLC demonstrated that the main composition of ginger oleoresin had no change before and after being included byβ-CD. CONCLUSION The inclusion complex with high inclusion rate can be obtained under the conditions of optimized preparation parameters.

关 键 词：姜油树脂/β-CD包合物 包合率 均匀设计 表征 

分 类 号：R944[医药卫生—药剂学]