TFPI-2基因对胰腺癌Panc-1细胞增殖和侵袭能力的影响  

Effect of TFPI-2 gene on cell proliferation and invasive ability of pancreatic cancer Panc-1 cell

作　　者：孙振阳[1] 汤志刚[1] 胡何节[1] 许戈良[1] 陈炯[1] 李建生[1] 

机构地区：[1]安徽医科大学附属安徽省立医院,安徽合肥230001

出　　处：《山东医药》2007年第21期21-23,共3页Shandong Medical Journal

基　　金：安徽省自然科学基金资助项目(050430702)

摘　　要：目的探讨外源性人组织因子途径抑制物（TFPI）-2基因表达对胰腺癌Panc-1细胞增殖和侵袭转移能力的影响。方法将人TFPI-2基因转染胰腺癌Panc-1细胞。用RT-PCR、Western blot技术检测目的基因的表达,用细胞生长试验、流式细胞仪分析技术以及Boyden小室法检测Panc-1细胞转染前后细胞增殖、细胞周期、细胞凋亡和细胞体外侵袭能力的变化。结果TFPI-2基因能在Panc-1细胞中稳定表达;与对照组相比,转染后的Panc-1细胞生长受到抑制,阻滞于G0-G1期,早期细胞凋亡率增加,细胞体外侵袭能力受到抑制。结论TFPI-2基因表达能够在体外抑制胰腺癌细胞系Panc-1的细胞增殖,降低其体外侵袭转移能力。[Objective] To evaluate the effect of TFP1-2 gene on cell proliferation and invasive ability of hmnan pancreatic cancer cell line Pane-1. [Methods]TFP1-2 gene was transfected into Pane- 1 cell, TFPI-2 mRNA and protein expression were detected by RT - PCR and Western blot. MTT assay was used to assess the effect of TFPI - 2 on cell proliferation. The change of cell cycle and apoptosis were measured by flow cytometry and the invasive ability in vitro was detected by Boyden chamber. [ Results ] RT - PCR and Western blot analysis showed that Panc - 1 cell could express TFPI - 2 stably. Compared with non - transfected cells, the growth of Panc - 1 cell transfected with TFPI - 2 was inhibited significantly. The transfected cells showed a significant increase in GO - G1 phase and could be induced to apoptosis of in comparison with the control group. The invasive ability of Panc - 1 cell was inhibited by expression of TFP1-2 effectively. [Conclusions] The expression of TFPI - 2 can inhibit cell proliferation and invasive ability of human pancreatic cancer cell line Panc - 1.

关 键 词：胰腺肿瘤 细胞增殖 肿瘤浸润 组织因子途径抑制物2 

分 类 号：R735.9[医药卫生—肿瘤]