IL-6 mRNA和TGFβ_1 mRNA在慢性根尖周病损组织中的表达  

Expression of IL-6 mRNA and TGFβ_1 mRNA in chronic periapical lesions

作　　者：杨文东[1] 孙建军[1] 罗顺云[1] 李志新[2] 高学军[3] 

机构地区：[1]中国医学科学院中国协和医科大学北京协和医院口腔科,北京100730 [2]北京大学医学部基础医学院中西医结合教研室 [3]北京大学口腔医学院.口腔医院牙体牙髓科

出　　处：《北京口腔医学》2007年第4期193-196,共4页Beijing Journal of Stomatology

摘　　要：目的检测IL-6 mRNA和TGFβ1 mRNA在慢性根尖周病损中的表达及它们与临床病理表现之间的关系,探讨IL-6和TGFβ1在慢性根尖周炎发病中的作用。方法采用RT-PCR法,从mRNA水平检测IL-6和TGFβ1在10例慢性根尖肉芽肿及3例根尖囊肿中的表达,3例阻生拔除的第三磨牙牙周膜组织作为对照,分析IL-6 mRNA和TGFβ1 mRNA的表达水平与慢性根尖周病损的病理学表现之间的关系,并分析IL-6 mRNA和TGFβ1 mRNA与病损大小之间的关系。结果IL-6 mRNA和TGFβ1 mRNA在根尖肉芽肿病损组和根尖囊肿组明显增高,表现为与β-actin水平的比值增高,IL-6 mRNA和TGFβ1 mRNA在根尖肉芽肿组和根尖囊肿组中分别为1.11±0.24和0.87±0.10及0.94±0.53和1.55±0.22。TGFβ1在根尖囊肿组及大的根尖周病损中有增高的趋势。TGFβ1 mRNA在中度、重度炎症细胞浸润组较轻度炎症细胞浸润组明显增高(P<0.05)。根尖周病损中的IL-6 mRNA和TGFβ1 mRNA水平未发现有明显的相关关系。结论IL-6和TGFβ1在慢性根尖周炎的发病中可能具有重要的作用。Objective To investigate the expression of IL-6 mRNA and TGFβ1 mRNA in chronic periapical lesions and their relations to the histological findings of the lesion. Methods A total of 13 periapical lesions including 10 periapical granulomas and 3 periapical cysts were obtained. Normal periodontal ligament tissues from extracted third molors were used as control. The expression of IL-6 mRNA and TGFβ1 mRNA was detected by reverse transcription polymerase chain reaction （ RT-PCR ） technique. The relationship between the expression of IL-6 mRNA and TGFβ1 mRNA and histological findings （including the type of histology, the degree of inflammatory infiltrates） and the size of radiolucency of the lesions was analyzed. Results A significantly higher level of IL-6 mRNA and TGFβ1 mRNA was found in periapical granulomas and in periapical cysts , the level of TGFβ1 mRNA tended to be higher in periapical cysts than in periapical granulomas. TGFβ1 mRNA in the group with moderate and heavy degree of inflammatory cell infiltration was higher than that in the group with light degree of inflammatory cell infiltration. Higher levels of TGFβ1 mRNA were detected in large lesions than in small lesions, and the level of IL-6 mRNA was not significantly different between the size of the lesions and the degree of inflammatory infiltration. Conclusion IL-6 and TGFβ1 might play an important role in the pathogenesis and development of periapical lesions.

关 键 词：IL-6 TGFΒ1 根尖周病损 

分 类 号：R781.34[医药卫生—口腔医学]