吡格列酮治疗STZ诱导糖尿病大鼠视网膜中TSP-1的变化  

THE CHANGES OF TSP-1 EXPRESSION IN THE RETINA OF STZ-INDUCED RAT DIABETIC MELLITUS MODEL WITH PIOGLITAZONE

作　　者：张惠燕[1] 王剑勇[2] 张晓明[3] 沈方[3] 

机构地区：[1]杭州职业技术学院,杭州310018 [2]浙江大学医学院附属第一医院,杭州310003 [3]浙江大学医学院,杭州310006

出　　处：《分子细胞生物学报》2007年第4期199-204,共6页Journal of Molecular Cell Biology

基　　金：浙江省教育厅科研基金(No.050309G20512)。~~

摘　　要：观察吡格列酮在治疗STZ诱导糖尿病大鼠视网膜中血小板反应素Ⅰ(thmmbospondin-1,TSP-1)的变化,探讨噻唑烷二酮类药物在糖尿病视网膜病变中的影响和可能作用机制。用链脲佐菌素(STZ)腹腔注射建立大鼠糖尿病模型,随机分正常对照组(C组),糖尿病安慰剂组(D组)和糖尿病吡格列酮治疗组(DP组),8周后取视网膜组织行免疫组织化学、RT-PCR半定量检测TSP- 1mRNA水平的变化,结果显示在糖尿病早期视网膜神经节细胞层、内核层中均有明显的TSP1表达,对比于糖尿病对照组,糖尿病治疗组视网膜单位面积表达TSP1的细胞数量要较二组对照组高;糖尿病治疗组、糖尿病对照组和正常对照组间TSP-1的荧光灰度值分别为528.2±47.78、493.4±39.36和417.7±28.74:内参照GAPDH的荧光灰度值分别为796.4±21.18、811.2±36.80和832.4±43.18,各组间TSP-1 mRNA表达有显著性差异,提示吡格列酮对糖尿病早期视网膜中TSP-1的表达变化有影响,吡格列酮可能在延缓糖尿病视网膜病变的发生发展中起一定的作用。To observe the changes of TSP-1 expression in the retina of STZ-induced rat diabetic mellitus model with pioglitazone and to elucidate the possible mechanism involved and the effects of thiazolidinediones compound pioglitazone on the early stages of diabetic retinopathy. 30 Male SD rats were randomly divided into 3 groups： control group, DM group and pioglitazone treated DM group （DP group）. DM group and pioglitazone treated DM group were injected with STZ intraperitoneally. 8 weeks after the onset of diabetes, the expression of TSP1 mRNA was quantified in retinal tissue by quantitative reverse transcription-polymerase chain reaction （RT- PCR） respectively. The locations of TSP-1 in three groups were also established by immunohistochemistry. 8 weeks after the onset of diabetes, every layer of the retina tissue had significant TSP- 1 positive staining and when compared with the control group and DM group, the pioglitazone treated DM group had higher TSP-1 positive cell number per unit area; There were significant differences among the DM group, pioglitazone treated DM group and control group in TSP-1 mRNA level, DM group and pioglitazone treated DM group had significantly higher TSP-1 mRNA level than the control group. Our results showed that pioglitazone treatment can alleviate the pathological changes of DR and the expression level of TSP-1 was increased in pioglitazone treated DM rats compared with other DM rats. Our study suggests pioglitazone may have a role on early stage of DR by increased TSP-1 expression.

关 键 词：吡格列酮 糖尿病视网膜病变 链脲佐菌素 血小板反应素Ⅰ 

分 类 号：R587.1[医药卫生—内分泌] R774.1[医药卫生—内科学]