微波辅助提取牡丹皮中的酪氨酸酶抑制剂  

Microwave-assisted Extraction of Tyrosinase Inhibitor from Paeonia suffruticosa

作　　者：龚盛昭[1] 杨卓如[2] 张木全[1] 

机构地区：[1]广东轻工职业技术学院轻化工程系,广东广州510300 [2]华南理工大学化工学院,广东广州510640

出　　处：《精细化工》2007年第8期766-768,777,共4页Fine Chemicals

基　　金：广东省佛山市产学研专项资金项目(2003A36)~~

摘　　要：以提取物产率及其对酪氨酸酶的相对抑制率为评价指标,进行了微波辅助提取牡丹皮中酪氨酸酶抑制剂的工艺研究。得到了最佳提取工艺条件:牡丹皮粒度为0.45 mm,以体积分数为70%的乙醇为提取剂,乙醇与牡丹皮的质量比为8∶1,微波450 W提取6 min,重复提取2次。提取液经活性炭脱色、真空浓缩、真空干燥后得浸膏,产率约22.8%,对酪氨酸酶的相对抑制率为82.8%。与直接加热提取法进行了比较,结果表明,微波辅助提取时间由90 min缩短至6 min,提取物产率由19.7%提高至22.8%,对酪氨酸酶的相对抑制率由77.2%提高至82.8%。The microwave-assisted extraction （MAE）technology of tyrosinase inhibitor from Paeonia suffruticosa was studied with the yield and the relative inhibitory ratio to tyrosinase （RIRT） of the extract as evaluating indexes. The optimal technological conditions were obtained as follows. Paeonia suffruticosa was crushed to 0.45 mm and extracted twice extractant （mass ratio of ethanol to Paeonia suffruticosa （each time 6 min） using 70% ethanol as 8 ：1 ） with the cooperation of microwave （microwave power 450 W ）. After the extracted liquor was decoloured with activated carbon, concentrated under vacuum, and then dried under vacuum, dry extract of Paeonia suffruticosa was obtained in 22. 8 % yield and the RIRT was 82.8 %. Compared with direct-heating extraction （DHE） ,MAE can shorten the extraction time from 90 min to 6 min,improve the yield from 19. 7% to 22. 8% ,and RIRT from 77.2% to 82. 8%.

关 键 词：牡丹皮 微波辅助提取 酪氨酸酶抑制剂 中药 丹皮酚 

分 类 号：TQ464.7[化学工程—制药化工]