含人铜锌SOD基因逆转录病毒重组体的构建  

CONSTRUCTION OF A RETROVIRAL RECOMBINANT CONTAINING HUMAN CuZn-SOD GENE

作　　者：郑新程[1] 安威[2] 伍贻经[1] 

机构地区：[1]北京医科大学病理生理学系,100083 [2]首都医科大学细胞生物学系

出　　处：《北京医科大学学报》1997年第2期112-114,共3页Journal of Peking University(Health Sciences)

摘　　要：目的：构建含人铜锌SOD基因逆转录病毒重组体。方法：通过两步克隆将编码人铜锌超氧化物歧化酶（hSOD1）基因片断从表达载体Bluescriptll中切下，定向插入逆转录病毒载体XM-6上，以限制性内切酶切分析法判定插入是否正确。结果：限制性内切酶谱分析与理论计算相符。结论：含人铜锌SOD基因逆转录病毒重组体构建成功，为今后进行心肌保护、抗动脉粥样硬化及抗衰老的基因治疗等开辟了广阔的前景。Objective: To construct a retroviral recombinant containing human CuZn-SOD gene. Methods:With two steps of molecular clonging, a fragment coding human copper/zinc superoxide dismutase(hSOD1) gene was digested from recombinant BluescriptII/SOD(+), and inserted into the vetroviralvector XM-6. The positive clonies were analysized by using restriction mapping. Results: The structureis proved right by restriction mapping. Conclusion: A retroviral recombinant containing hSOD1 genewas obtained. Its expression and gene therapy used for ischemia, arteriosclerosis and aging are underway.

关 键 词：逆转录病毒 基因 超氧化物歧化酶 铜 锌 重组体 

分 类 号：R394－33[医药卫生—医学遗传学]