用DNA指纹技术检测儿童急性粒细胞白血病的基因重排  

DETECTION OF GENE REARRANGEMENT OF ACUTE MYELOID LEUKEMIA IN CHILDREN WITH DNA FINGERPRINTING

作　　者：王黛[1] 辛德丽[2] 张小为 史秀芬 

机构地区：[1]北京医科大学人民医院医学遗传科,100034 [2]北京市友谊医院儿科 [3]北京市刑事科学技术研究所

出　　处：《北京医科大学学报》1997年第2期129-130,133,共3页Journal of Peking University(Health Sciences)

摘　　要：目的：应用DNA指纹分析技术检测儿童急性粒细胞白血病的基因重排。方法：用LE11．8、MYO和M13探针，经Southern杂交法检测12例儿童急性粒细胞白血病患者的外周血或骨髓细胞的基因重排。结果：发现初治或复发与完全缓解时的DNA指纹图相比，谱带有增加或减少。结论：急性粒细胞白血病患儿的白血病细胞存在基因重排；DNA指纹可以区别白血病细胞和正常细胞；DNA指纹技术可以检测到残留的少量白血病细胞。Objective: To investigate the gene rearrangement of children in acute myoploid leukemia(AML) by DNA fingerprints. Methods: The gene rearrangement in blood and bone marrow of 12 children with AML was detected by Southern blot hybridization using LE11. 8, MYO and M13 probes. Results: The bands of DNA fingerprints at the time of diagnosis or relapse compared with remission weredifferent, which were increased or reduced. Conclusions: There is gene rearrangement in the leukemiacells of AML in children. DNA fingerprints can distinguish leukemia cells from normal cells. DNA fingerprints can detect minimal residual leukemia cells. DNA fingerprints prove to be a sensitive method inthe detection of minimal residual disease of AML and provide a new way for studying molecular pathology of leukemia.

关 键 词：DNA指纹 基因重排 急性白血病 儿童 

分 类 号：R733.710.2[医药卫生—肿瘤]