冻融法提取猪血纤维蛋白原  

Extraction of fibrinogen from pig blood by freeze-thawing

作　　者：李敏康[1] 钱冬明[1] 

机构地区：[1]陕西科技大学生命科学与工程学院,西安710021

出　　处：《分析试验室》2007年第4期65-68,共4页Chinese Journal of Analysis Laboratory

基　　金：陕西省教育厅专项(JK05-145)项目资助

摘　　要：利用纤维蛋白原在低温下可形成絮状沉淀的性质,以样品中纤维蛋白原含量及提取率为考查指标,采用冻融法从猪血中提取纤维蛋白原。确定提取条件为:将抗凝血浆在-20℃温度下冷冻12h,经4℃解冻,低温离心收集纤维蛋白原样品。样品中纤维蛋白原含量达82.6%,提取率达91.0%,符合静脉注射纤维蛋白原要求。　A method for the extraction of fibrinogen from pig blood by freezing and thawing was developed,based on deposition of fibrinogen under low temperature,as well as using the content of fibrinogen in samples and extraction efficiency as indexes.The extracting conditions for fibrinogen were as follows：the blood plasma was frozen about 12 h under the temperature of-20 ℃ with addition of anticoagulant,and thawed at 4 ℃,and then fibrinogen was collec-ted with centrifugation at low temperature.The concentration of fibrinogen is 82.6% and the extraction efficiency is 91.0%.The product satisfies the demand of mainline.

关 键 词：纤维蛋白原 冷冻 融化 提取率 

分 类 号：O652.6[理学—分析化学]