荧光PCR和PFGE在副溶血弧菌食物中毒诊断中的应用研究  

APPLICATION OF REAL-TIME PCR AND PULSED-FIELD GEL ELECTROPHORESIS IN THE DIAGNOSIS OF VIBRIO PARAHAEMOLYTICUS FOOD POISONING

作　　者：兰全学[1] 李庆阁[2] 扈庆华[1] 石晓路[1] 王冰[1] 林一曼[1] 

机构地区：[1]深圳市疾病预防控制中心,深圳518020 [2]厦门大学

出　　处：《现代预防医学》2007年第16期3001-3002,共2页Modern Preventive Medicine

基　　金：国家自然科学基金资助项目(30300281);广东省卫生厅资助项目(A2003709)

摘　　要：[目的]探讨荧光PCR和脉冲场电泳(PFGE)在副溶血弧菌食物中毒调查中的应用。[方法]对中毒样品进行荧光PCR检测,副溶血弧菌临床分离株基因组DNA经NotI酶切,通过PFGE获得电泳图谱,利用BioNumerics软件对图谱进行聚类分析。[结果]部分样品荧光PCR检测结果呈阳性,同起食物中毒菌株PFGE图谱一致。[结论]荧光PCR可以快速准确的鉴定病原体。PFGE具有很强的菌株同源性分析能力,适用于食物中毒病原菌学鉴定和传染源溯源。荧光PCR和PFGE在食物中毒的快速诊断中可发挥重要作用。[Objective] To explore the application of real-time PCR and pulsed-field gel electrophoresis （PFGE） in the investigation of Vibfio parabaemolyticus food poisoning, [Methods] The poisoning samples were assayed by real-time PCR. Vibrio parahaemolyticus strains were analyzed by pulsed-field gel electrophoresis with restriction enzyme NotI. PFGE patterns of isolates were clustered using BioNumerics software. [ Results] Some samples were positive using real-time PCR assay. PFGE patterns of strains from the same outbreak of food poisoning were identical. [Conclusions] Real-time PCR can be applied in the rapid detection of Vibrio parabaemolyticus. PFGE has high resolution, which enables itself to identify pathogen and trace in- fection sources. Real-time PCR and PFGE should play important roles in food poisoning diagnosis.

关 键 词：荧光PCR 脉冲场电泳 副溶血弧菌食物中毒 

分 类 号：R155.31[医药卫生—营养与食品卫生学]