声诺维联合超声辐照对RPE细胞增殖的影响  

Impact of Ultrasound Exposure Combined with SonoVue on Retinal Pigment Epithelium Proliferation

作　　者：伍瑛[1] 杜联芳[1] 顾青[2] 

机构地区：[1]上海交通大学附属第一人民医院超声科,上海市200080 [2]上海交通大学附属第一人民医院眼科研究室,上海市200080

出　　处：《中国超声医学杂志》2007年第8期561-563,共3页Chinese Journal of Ultrasound in Medicine

基　　金：上海市自然科学基金(No.05ZR14087)

摘　　要：目的探讨微泡造影剂声诺维(SonoVue)联合超声辐照对RPE细胞增殖的影响。方法在96孔板中,对照组不处理,实验组分为超声声强、辐照时间、造影剂浓度、超声形式组处理,MTT法检测对RPE细胞增殖的影响,计算细胞存活率。结果实验组的吸光度值较对照组有不同程度的降低,各组间差异有显著性意义(P=0.0001)。第4、6、8、9、10组与第1组比差异有显著性意义(P<0.05)。造影剂浓度增加对细胞增殖有明显抑制作用(P<0.05)。结论一定剂量的微泡造影剂联合超声辐照对RPE细胞增殖有抑制作用。Objective To investigate whether ultrasound mediated microbubbles destruction could impact on the proliferation of cultured retinal pigment epithelium （RPE） in vitro. Methods RPEs were cultured in 96 wells and exposed to ultrasound in different intensity, forms, radiation and different concentration of SonoVue in the experimental groups, but no treatment was applied to the control group. RPE proliferation was observed on MTT assay and the cell survival rate were calculated. Results The absorbed optical density of the experimental group was lower than that of the control group and the difference in the intergroups was significant （P = 0. 0001 ）. The difference of 4, 6, 8, 9, 10 vs the first were significant （P〈0. 05） . The increase of contrast agent concentration would inhibit cell proliferation obviously （P〈0. 05） . Conclusions Ultrasound mediated microbubble destruction could impact on the proliferation of RPE.

关 键 词：超声造影剂 人视网膜色素上皮细胞 细胞增殖 

分 类 号：R363[医药卫生—病理学]