JAK2激酶抑制剂AG490对结肠癌HT-29细胞侵袭性的影响  

Effect of AG490 on invasiveness of HT-29 cells

作　　者：许峰[1] 王杉[1] 叶颖江[1] 杨燊[1] 崔志荣[1] 

机构地区：[1]北京大学人民医院胃肠外科外科肿瘤实验室,100044

出　　处：《中华实验外科杂志》2007年第8期908-909,共2页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金(30271269)

摘　　要：目的观察JAK2激酶抑制剂AG490对结肠癌HT-29细胞侵袭以及对基质金属蛋白酶-2(MMP-2)表达的影响,探讨STAT3信号转导通路在结肠癌侵袭转移调控中的作用。方法应用JAK2激酶抑制剂AG490处理结肠癌HT-29细胞,Matrigel肿瘤体外侵袭模型检测肿瘤细胞侵袭;Western blot检测STA33蛋白表达;逆转录-聚合酶链反应(RT-PCR)检测MMP2 mRNA表达。结果ACA90可抑制JAK2/STA33信号转导通路活化及结肠癌HT-29细胞侵袭,在此过程中AG490在mRNA水平抑制MMP-2表达。结论STA33信号转导通路参与结肠癌细胞侵袭调控,阻断STA33通路活化可抑制结肠癌细胞侵袭,这一作用可能是通过抑制MMP-2表达实现的。Objective To investigate the role of STAT3 signaling pathway in colorectal cancer invasiveness. Methods Janus kinase 2 selective inhibitor AG490 was used to inhibit the activation of STAT3 signaling pathway. Matrigel-coated transwell was employed to estimate the invasiveness of HT-29 cells. The changes in the activity of STAT3 and the expression of MMP-2 were measured by Western blot and RT-PCR respectively. Results The activation of STAT3 signaling pathway was suppressed by AG490, and at the same time, AG490 significantly decreased the invasiveness of colorectal cancer cell HT- 29 through Matrigel-coated filters. Decreased invasiveness was associated with the inhibition of MMP-2 at mRNA level. Conclusion Invasiveness of colorectal cancer cells may be suppressed by inhibition of STAT3 signaling pathway through down-regulating MMP-2.

关 键 词：结肠肿瘤 金属蛋白质酶 

分 类 号：R735.35[医药卫生—肿瘤]