寡核苷酸芯片筛查孤雄完全性葡萄胎患者的相关基因  

Oligonucleotide array of genomic expression in complete androgenic hydafidiform mole

作　　者：徐建云[1] 叶枫[1] 吕卫国[1] 洪蝶[1] 钱建华[1] 谢幸[2] 

机构地区：[1]浙江大学医学院附属妇产科医院妇瘤科,杭州310006 [2]江大学医学院附属妇产科医院妇瘤科,杭州浙310006

出　　处：《中华妇产科杂志》2007年第8期537-541,共5页Chinese Journal of Obstetrics and Gynecology

基　　金：国家自然科学基金(30471811)

摘　　要：目的在全基因组 DNA 水平探讨孤雄完全性葡萄胎患者的相关基因,并探索其发生的分子机制。方法短串联重复序列(STR)基因位点鉴定孤雄完全性葡萄胎的亲代来源;人基因组U133 Plus 2.0寡核苷酸芯片检测孤雄完全性葡萄胎和正常早孕绒毛组织中全基因组 DNA 水平的差异表达基因,荧光定量 RT-PCR 技术验证小部分差异表达基因;对寡核苷酸芯片检测的数据进行生物信息学分析。结果 11例经病理检查确诊的完全性葡萄胎患者中,其基因组 DNA 经 STR 基因位点鉴定显示,9例(82%)为父系来源,2例(18%)为双亲来源;寡核苷酸芯片检测显示,和正常早孕绒毛组织相比,孤雄完全性葡萄胎组织中表达上调基因279个,占检测基因的0.72%(279/38500),表达下调基因1710个,占检测基因的4.44%(1710/38500);小部分差异表达基因的荧光定量 RT-PCR 技术检测与寡核苷酸芯片检测结果一致;生物信息学分析显示,差异表达基因涉及多个生物学过程和通路,其中印迹基因的变化和生长激素及人胎盘生长催乳激素基因的变化尤为明显。结论孤雄完全性葡萄胎的发生、发展是涉及多基因、多途径的复杂过程,其中印迹基因和生长激素类基因表达改变起重要作用。Objective To compare genomic expression differences between androgenic complete hydatidiform mole （AnCHM） and normal first trimester villi with similar gestation weeks, and search for potential adjuvant diagnostic molecular markers. Methods Short tandem repeat （STR） detection was used to identify AnCHM, human oligonucleotide array U133 Plus ：2. 0 was used to measure genomic expression differences between AnCHM and normal villi, and quantitative fluorescent RT-PCR was used to verify array of several genes. Results Nine of 11 histologically diagnosed complete hydatidiform moles were found to be AnCHM by means of STR, and the other ：2 were biparental complete hydatidiform mole （BiCHM）. Compared with villi, oligonucleotide array showed ：279 genes （0. 72% , ：279/38 500） were over expressed and 1710 genes （4.44%, 1710/38 500） under expressed in AnCHM. Bioinformatics analysis found that differentially expressed genes were involved in multiple biological processes and pathways. Changes of imprinting genes, growth hormone genes and chorionic somatomammotropin hormone genes were especially remarkable. Conclusions Pathogenesis of AnCHM is a complex process involving multiple genes and pathways. Altered expression of imprint genes may play important roles in the process.

关 键 词：葡萄胎 寡核苷酸序列分析 串联重复序列 

分 类 号：R737.33[医药卫生—肿瘤]