p53基因敲除对细胞迁移的影响  

Effect of p53 gene knockout on cell migration

作　　者：龚小卫[1] 魏洁[1] 李煜生[1] 程蔚蔚[1] 王旭[1] 邓鹏[1] 姜勇[1] 

机构地区：[1]南方医科大学病理生理学教研室和广东省蛋白质组学重点实验室,广东广州510515

出　　处：《南方医科大学学报》2007年第8期1132-1135,共4页Journal of Southern Medical University

基　　金：广东省自然科学基金重点项目(13058);广东省科技计划项目(A1090202);广州市科技计划项目(2001-z-035-01-1)~~

摘　　要：目的研究p53基因敲除对细胞迁移的影响。方法观察p53+/+和p53-/-细胞中血清诱导的板状伪足形成情况,以及利用Transwell细胞迁移系统观察p53基因敲除对血清诱导的细胞迁移的影响。结果在血清诱导下,p53+/+细胞能形成板状伪足和进行迁移,而p53-/-细胞中板状伪足的形成和细胞迁移都被阻断。结论p53在血清诱导的细胞迁移中具有重要作用。Objective To investigate the role of p53 gene in serum-induced cell migration, Methods The effects of p53 knockout on serum-induced formation of lamellipodia and cell migration were observed using Transwell cell migration system, Results p53^＋/＋ cells developed lamellipodia upon serum stimulation and showed enhanced activity of cell migration, but these effects were not observed in p53 knockout cells after serum stimulation, Conclusion p53 plays a role in serum-induced cell migration.

关 键 词：P53 基因敲除 细胞迁移 

分 类 号：Q343[生物学—遗传学]