辐射诱导pcDNA3.1-Egr.1p-p16对JF305细胞周期和凋亡的影响  

Effect of irradiation-induced pcDNA3.1-Egr.1p-p16 on cell apoptosis and cell cycle of JF305 cells

作　　者：马红兵[1] 王西京[1] 马洁[2] 夏辉[2] 王铮[2] 李琤[2] 韩志楷[2] 吴丛梅[3] 

机构地区：[1]西安交通大学医学院第二医院肿瘤科,陕西西安710004 [2]中国医学科学院肿瘤研究所分子肿瘤学国家重点实验室,北京100021 [3]汕头大学医学院生物教研室,广东汕头515031

出　　处：《南方医科大学学报》2007年第8期1183-1186,共4页Journal of Southern Medical University

基　　金：陕西省科技攻关项目(2002K10-G3)~~

摘　　要：目的检测pcDNA3.1-Egr.1p-p16质粒对胰腺癌细胞JF305凋亡和细胞周期变化的影响。方法进行人胰腺癌JF305细胞培养,脂质体LipofectamineTM2000转染pcDNA3.1-Egr.1p-p16重组质粒,6MV-X射线4Gy照射(剂量率2.50Gy/min),流式细胞术检测细胞周期和细胞凋亡状况。结果pcDNA3.1-Egr.1p-p16质粒转染组和pcDNA3.1-Egr.1p-p16质粒转染+4Gy照射组的JF305细胞中早期凋亡细胞分别占6.4%、10.4%,晚期凋亡或继发性死亡细胞分别占16.8%、33.8%,均较阴性对照组显著升高(P<0.05)。质粒转染+4Gy照射组总的凋亡率高于单纯质粒转染组(P<0.05)。辐射明显导致JF-305细胞G2期阻滞。pcDNA3.1-p16质粒和pcDNA3.1-Egr.1p-p16质粒基因转染,可使JF-305细胞阻滞于G1期。当给予细胞4Gy照射后,两质粒转染组,阻滞于G1期细胞变化不大,阻滞于G2期的细胞有所增加。结论pcDNA3.1-Egr.1p-p16可致JF-305细胞凋亡,与放射联合增加了细胞凋亡率。转染pcDNA3.1-Egr.1p-p16可使细胞阻滞于G1期,联合辐射,增加了细胞的G2期阻滞。Objective To observe the effect of pcDNA3.1-Egr, 1p-p16 plasmid on apoptosis and cell cycle of pancreatic carcinoma JF305 cell line. Methods JF305 cells were cultured and transfected with pcDNA3.1-Egr.1p-p16 plasmid via LipofectamineTM 2000, followed by irradiation by 6MV-X at 4 Gy （dose rate 2.50 Gy/min）. The cell cycle and cell apoptosis changes were analyzed by flow cytometry. Results In cells infected with pcDNA3.1-Egr.1p-p16 plasmid and those with pcDNA3.1-Egr, 1p-p16 plasmid infection before 4 Gy irradiation, the percentages of viable apoptotic cells were 6.4% and 10.4%, and those of advanced stage apoptotic or dead cells were 16.8% and 33.8%, significantly higher than those in the control group （P〈0.05）. JF305 cell apoptosis in 4 Gy irradiation group was obviously increased in comparison with non-irradiatedplasmid-infectedcells （P〈0.05）. IrradiationresultedinapredominantG2arrestoftheplasmid-infectedJF305 cells. Both pcDNA3.1-p 16 plasmid and pcDNA3.1-Egr. 1 p-p 16 plasmid infections could induce G1 arrest of JF-305 cells, and irradiation did not produce significant changes in G1-arrested cells in the two plasmid infection groups, but cells arresting at G2 phase increased. Conclusion pcDNA3.1-Egr,1 p-p 16 can induce JF-305 cells apoptosis, which is enhanced by irradiation. pcDNA3.1-Egr, lp-p16 tranfection may result in G1 arrest of the cells, and when combined with irradiation, the cells arrested at G2 phase can increase.

关 键 词：胰腺癌 质粒 细胞周期 凋亡 

分 类 号：R735.9[医药卫生—肿瘤]