日本刺参胶原肽对B16黑素瘤细胞黑素合成的影响  

EFFECT OF COLLAGEN POLYPEPTIDES FROM APOSTICHOPUS JAPONICUS ON MELANOGENESIS IN B16 MELANOMA CELLS

作　　者：王奕[1] 王静凤[1] 张瑾[1] 崔凤霞[1] 薛长湖[1] 

机构地区：[1]中国海洋大学食品科学与工程学院,青岛266003

出　　处：《营养学报》2007年第4期401-404,共4页Acta Nutrimenta Sinica

基　　金：国家自然科学基金(No.30471319);新世纪优秀人才支持计划项目(No.NCET-04-0642)

摘　　要：目的:研究不同分子量日本刺参胶原肽A1(6000U<Mr<10000U)、A2(Mr<6000U)对小鼠B16黑素瘤细胞黑素生成、酪氨酸酶活性及酪氨酸酶基因表达的影响。方法:采用MTT法测定细胞增殖活性,NaOH裂解法测定黑素生成量,多巴氧化法测定酪氨酸酶活性,逆转录-聚合酶链反应(RT-PCR)测定酪氨酸酶mRNA表达。结果:不同分子量日本刺参胶原肽均能显著促进B16细胞增殖,抑制B16细胞黑素合成和酪氨酸酶活性,下调酪氨酸酶mRNA表达,且剂量效应关系明显。结论:不同分子量日本刺参胶原肽均具有显著抑制B16黑素瘤细胞黑色合成的作用。其中,A1的抑制效果较A2显著。Objective： To study the effects of different molecular weight of collagen polypeptides from Apostichopus japonicus （A1：6 000U 〈Mr〈10 000U, A2：Mr〈6 000U） on melanogenesis, trosinase activity and mRNA expression in B16 melanoma cells. Method： Cell proliferation rate was detected by MTT assay, the tyrosinase activity by dopa oxidase assay, the melanin content by NaOH assay, and the mRNA level of tyrosinase by RT-PCR assay. Results： The proliferation rate of B16 cells was stimulated significantly, and melanogenesis, tyrosinase activity were inhibited remarkably, and the tryrosinase mRNA level was decreased evidently in dose dependent manner by both A1 and A2 respectively. Conclusion： Different molecular weight of collagen polypeptides from Apostichopus japonicus can inhibit melanogenesis in B16 melanocytes significantly, A1 better than A2.

关 键 词：日本刺参胶原肽 B16黑素瘤细胞 黑素合成 酪氨酸酶活性 MRNA表达 

分 类 号：R285.5[医药卫生—中药学] R739.5[医药卫生—中医学]