酚酸类物质对水华藻类的化感作用及其机理  

The allelopathy and its mechanism of phenolic acids on water-bloom algae

作　　者：张庭廷[1] 吴安平[1] 何梅[1] 陈传平[1] 聂刘旺[1] 

机构地区：[1]安徽师范大学生命科学学院,生物环境与生态安全安徽省高校重点实验室,安徽芜湖241000

出　　处：《中国环境科学》2007年第4期472-476,共5页China Environmental Science

基　　金：安徽省教育厅自然科学基金资助项目(2006KJ219B)

摘　　要：研究了酚酸类物质阿魏酸和对羟基苯甲酸对水华鱼腥藻(Anabaena flos-aquae)以及蛋白核小球藻(Chlorella pyrenoidosa)的化感抑制作用及其机理.结果表明,对羟基苯甲酸浓度为0.6mmol/L,阿魏酸浓度为1.0mmol/L时,对水华鱼腥藻和蛋白核小球藻均有明显的抑制作用,其中对羟基苯甲酸的化感抑制作用较阿魏酸显著,对水华鱼腥藻的抑制作用比对蛋白核小球藻显著.其抑制作用表现为藻细胞生长量以及藻细胞叶绿素a含量下降、细胞内超氧阴离子自由基增高以及膜脂质过氧化产物丙二醛(MDA)积累、细胞超氧化物歧化酶(SOD)的活力和比活力开始时应激性升高.推测酚酸类物质化感抑制作用的机理可能与自由基的产生以及膜脂质过氧化增加引起膜结构的破坏、细胞功能受损有关.2种酚酸类物质联合用时具有协同抑藻效应.The allelopathic inhibition and its mechanism of ferulic acid and p-hydroxybenzoic acid on Anabaena flos-aquae and Chlorella pyrenoidosa were studied. When the concentrations of ferulic acid and p-hydroxybenzoic acid were 1.0mmol/L and 0.6mmol/L, there all had obvious inhibitory effect on Anabaena flos-aquae and Chlorella pyrenoidosa, in which the allelopathic inhibitory effects ofp-hydroxybenzoic acid were more marked than ferulic acid, the inhibitory effects on Anabaena flos-aquae were more marked than Chlorella pyrenoidosa. Its inhibitory effects showed that the growth amount and chlorophyll-a content of algal cell decreased, superoxygen negative ion free radical in cell enhanced, membrane lipid superoxidized product malondialdehyde （MDA） accumulated, the activity and specific activity of cell superoxide dismutase（SOD） were of emergency enhance in the beginning. The allelopathy and its inhibitory mechanism of phenolic acids were inferred relating positively with the producing of free radical, and the damage of membrane structure caused by increase of membrance lipid peroxidation and the suffering of cell function. When the two phenolic acids were used jointly, it possessed synergistic inhibiting effect.

关 键 词：酚酸 藻类 化感作用 生长抑制 化感机制 

分 类 号：X171[环境科学与工程—环境科学]