大鼠烟曲霉菌性角膜炎角膜局部的免疫学研究  

Study on immunopathogenesis of corneal tissues in experimental aspergillus fumigatus keratitis in rat

作　　者：崔红平[1] 苏晶[2] 高新蕊[1] 

机构地区：[1]同济大学附属第十人民医院眼科,上海200072 [2]上海中医药大学附属龙华医院眼科,上海200072

出　　处：《眼外伤职业眼病杂志》2007年第8期561-565,共5页Journal of Injuries and Occupational Diseases of the Eye with Ophthalmic Surgeries

摘　　要：目的探讨大鼠角膜真菌感染后角膜局部的免疫反应机制。方法模拟角膜外伤制作大鼠的烟曲霉菌性角膜炎模型,在感染后不同阶段进行病理切片和免疫组化观察角膜局部中性粒细胞、总T淋巴细胞及T淋巴细胞亚群、树突状细胞、B淋巴细胞的动态变化。结果在大鼠烟曲霉菌性角膜炎早期,见大量中性粒细胞及少量CD3+T、CD4+T、CD8+T淋巴细胞和LC浸润,未检测到B淋巴细胞。中期,中性粒细胞浸润达高峰,T淋巴细胞浸润增加,其中CD4+T淋巴细胞多于CD8+T淋巴细胞。树突状细胞亦增多,B淋巴细胞开始出现。晚期,中性粒细胞数目有所减少,T淋巴细胞浸润达高峰,CD4+T淋巴细胞亦多于CD8+T淋巴细胞,B淋巴细胞少量表达。结论免疫反应与真菌性角膜炎的病程变化有密切的关系。Objective To identify the locailzation and kinet-ics of infihratrated immunity cells in the corneas. Methods Estab-lished a rat model of Aspergillus fumigatus keratitis. Immunohisto- chemistry was carried out on the cornea pathology slice at different time points to detect the neutrophil, langerhans cell, T-lymphocytes and their subpopulation （ CD4^＋ , CD8^＋） and B-lymphocytes. Re- suits In the early stage of AFK （ 1-3 days after inoculated） ,there were a lot of neutrophil and a few of T-lymphocytes CD3^＋ , C4^＋, and CD8^＋ and langerhans cells,but no B-lymphocytes. At 4-7 days after inoculated, the ulcerls fried and compacted, the inflammation reached its peak stage. The fungal were in the former 1/3 - 2/3 cor- nea and were segregated by lots of immune cells. A massive influx of neutrophils and increasing T-lymphocytes and langerhans cells were seen in the ulcer and every layers of comes. CD4^＋ T-lympho- cytes were much more than the CD8^＋ T-lymphocytes in number. B-lymphocytes could also found. In the late stage （9-14 days after in-oculated） , we could see cornea leucoma and lots of neoveasculariz-tion. Neutrophils decreased but T-lymphocytes and langerhans cells were at a massive stage, CD4^＋ T-lymphocyte were also moe than CD8^＋ T-lymphocytes. And the B-lymphocytes were also increased. The changes of granulomatous inflammaation were seen in the slices but no fungi of spores. Conclusions Immunity take part in the rat fungal keratitis,especial in the late stage.

关 键 词：真菌性角膜炎 烟曲霉菌 免疫学 大鼠 

分 类 号：R772.21[医药卫生—眼科]