γ射线对小鼠主动脉性腺中肾区基质细胞氨基肽酶N／CD13活性的影响  

Effect of irradiation on APN/CD13 enzymatic activity on AGM stromal cells

作　　者：朱艳[1] 黄丽芳[1] 罗小华[1] 孙汉英[1] 冉丹[1] 张可杰[1] 郑邈[1] 周琨[1] 刘文励[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院血液内科,武汉430030

出　　处：《中华放射医学与防护杂志》2007年第4期307-310,共4页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金资助项目（30570773）

摘　　要：目的探讨小鼠主动脉性腺中肾（AGM）区基质细胞氨基肽酶N／CD13（APN／CD13）的表达和放射损伤前后该酶活性的变化及意义。方法采用免疫组织化学染色、RT-PCR检测AGM区基质细胞APN／CD13的表达；^60Coγ射线8．0Gy照射细胞，在不同的时间点用分光光度计检测APN／CD13的酶活性。结果AGM区基质细胞APN／CD13呈强阳性表达；放射损伤后酶活性呈一过性降低，继而升高，4h达高峰，24—48h基本恢复至照射前水平。结论APN／CD13在AGM区基质细胞上呈强阳性表达；在放射损伤后酶活性增高，可能是促进造血功能恢复的一种代偿机制。Objective To investigate the expression of APN/CD13 in mice embryo AGM stromal cells and the change of its enzymatic activity before and after irradiation. Methods The expression of APN/CD13 in AGM stromal cells was assayed by RT-PCR and immunohistochemistry. The stromal cells in AGM region were irradiated with 8.0 Gy of ^60Co γ-rays, and APN/CD13 enzymatic activity was measured by spectrophotometer at different time points. Results The APN/CD13 expression level was enhanced significantly in AGM stromal cells. The enzymatic activity of APN/CD13 decreased temporally post-irradiation injury, then increased to the highest level 4 hours post-irradiation, and it returned to the level of before irradiation 24 to 48 hours postirradiation. Conclusions The expression of APN/CD13 was remarkable in AGM stromal cells. The enzyme activity of APN/CD13 was temporally enhanced after irradiation, which might be one of the compensatory mechanisms to promote the hematopoietic recovery after irradiation.

关 键 词：氨基肽酶N/CD13 主动脉性腺中肾区 基质细胞 放射损伤 酶活性 

分 类 号：R[医药卫生]