^125I粒子照射诱导人前列腺癌细胞凋亡的实验研究  

Study on apoptosis of prostate cancer cell induced by ^125I seed irradiation

作　　者：廖安燕[1] 王俊杰[1] 赵勇[2] 王济东[1] 庄洪卿[1] 

机构地区：[1]北京大学第三医院肿瘤治疗中心,100083 [2]中国科学院动物研究所生物膜国家重点实验室

出　　处：《中华放射医学与防护杂志》2007年第4期311-313,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金资助项目（30572137）

摘　　要：目的探讨^125I粒子持续低剂量率照射诱导人前列腺癌细胞凋亡的作用机制。方法用DNA琼脂糖凝胶电泳和流式细胞仪分析^125I粒子持续低剂量率照射对PC3细胞的凋亡诱导作用，检测天冬酰胺特异酶切的半胱氨酸蛋白酶Caspase-3活性来观察其对PC3细胞的凋亡诱导途径，并通过间接免疫荧光技术检测其对Bcl-2表达的影响。结果DNA琼脂糖凝胶电泳可观察到清晰的“DNA梯度”，流式细胞仪检测^125I粒子持续低剂量率照射对PC3细胞具有明显的凋亡诱导作用。Caspase-3活性检测表明，Caspase-3活性无明显变化。流式细胞仪分析表明Bcl-2的表达随^125I粒子剂量的增加而下降。结论^125I粒子持续低剂量率照射可诱导PC3细胞凋亡，其诱导凋亡与Bcl-2表达有关，与Caspase-3活性无关。Objective To explore the mechanism of apoptosis induced by ^125I seed irradiation on PC3 cells, Methods Human prostate cancer cell line PC3 was treated by iradiation of ^125I （2,77 cGy/h） with various dose. Agarose gel electrophoresis of DNA and flows cytometry were used to detect the apoptosis of PC3 cells and indirect immunofluorescence assay was used to detect the expression of Bcl-2,The activity of Caspase-3 was measured by Caspase Colorlmetric Assay Kits, Results Apoptosis of PC3 cells could be efficiently induced by ^125I seed irradiation,The apoptotic peaks were found by flow cytometry and DNA ladder appeared on 1,8% agarose gel ,The activity of Caspase-3 on PC3 cells treated by ^125I seed irradiation was not changed significantly. Bcl-2 gene expression was down-regulated with the sample concentration increased, Conclusion ^125I irradiation can induce the apoptosis of PC3 cells and the mechanism of apoptosis is related with down regulation of Bcl-2 gene expression and is not related with Caspase-3 activity.

关 键 词：^125I放射性粒子 前列腺癌细胞 细胞凋亡 天冬酰胺特异酶切的半胱氨酸蛋 白酶 Bcl-2 

分 类 号：R737[医药卫生—肿瘤]