曲妥珠联合干扰素α-2b对肾癌细胞株的影响  

Influence of trastuzumab with Interferon α-2b on renal carcinoma cell

作　　者：王家菁[1] 胡志全[2] 闫志安[1] 杨非衡[1] 朱润[1] 谷现恩[1] 叶章群[2] 

机构地区：[1]北京市垂杨柳医院暨同济大学北京微创医院泌尿外科,北京100022 [2]华中科技大学同济医学院附属同济医院泌尿外科,湖北武汉430030

出　　处：《医学研究生学报》2007年第8期788-791,共4页Journal of Medical Postgraduates

基　　金：华中科技大学同济医学院科研基金资助项目(批准号:200449)

摘　　要：目的:了解曲妥珠(Trastuzumab)联合干扰素α-2b(IFN-α-2b)对肾癌细胞在体外的杀伤抑制作用,同时检测肾癌细胞在药物作用前后人表皮生长因子2(HER2)、细胞间黏附分子1(ICAM-1)的表达情况。方法:肾癌细胞系通过细胞培养技术进行培养,用单四唑(MTT)法和Western blot技术观察曲妥珠联合IFN-α-2b对该细胞系的抑制杀伤效果,进而运用链霉抗生物素-过氧化物酶免疫组化(S-P)法对HER2、ICAM-1表达情况进行测定。流式细胞仪检测用药前后细胞凋亡情况。结果:曲妥珠联合IFN-α-2b对肾癌细胞的生长抑制及黏附性呈时间和剂量依赖性。经药物处理后,HER2、ICAM-1表达差异明显(P<0.05)。结论:曲妥珠能有效抑制肾癌细胞的生长,对增加瘤细胞的黏附性也有一定影响。Objective：To study the influence of trastuzumab and IFN-α-2b on ACHN of renal carcinoma cell （RCC） lines in vitro and on the expression of human epidermal growth factor receptor-2 （HER2） and inter-cell adhensive molecule-1 （ ICAM-1 ）. Methods. ACHN cell line of RCC were cultured. The tetrazolium-based colorimetric assay and Western blotting were used to evaluate the growth inhibitory effect of trastuzumab and IFN-α-2b. S-P method was used to determine the expression of HER2, ICAM-1 of the cell line. Results：Trastuzumab associated with IFN-α-2b showed the inhibitory effect on growth and adhesiveness in RCC since 40 μg/L and 24 h in time-effective and dose-dependent manner. After treatment with trastuzumab and/or IFN-α-2b, the expression of HER2, ICAM-1 genes of RCC was changed（P 〈 0.05 ）. Conclusion：Trastuzumab and IFN-α-2b could inhibit the proliferation of RCC and enhence the adhesiveness of these ceils.

关 键 词：肾细胞癌 曲妥珠 干扰素 

分 类 号：R737.11[医药卫生—肿瘤]