桂皮酸诱导高转移人肺癌细胞恶性表型逆转和抑制侵袭作用的研究  

Reversion of Malignant Phenotypes of Human Lung Cancer Cells and Suppression of Its Invasive Ability by Cinnamic Acid

作　　者：黄炜[1] 黄济群[1] 

机构地区：[1]广州医学院肿瘤研究所,广州市510182

出　　处：《中国肿瘤临床》1997年第6期456-460,共5页Chinese Journal of Clinical Oncology

摘　　要：本文作者选用桂皮酸处理克隆化高转移人肺巨细胞癌（PGCL3）细胞，发现肿瘤细胞在形态、增殖速度、分裂指数、软琼脂集落形成能力和凝集反应等方面均出现向正常细胞表型逆转，证明桂皮醚具有抑制PGCL3细胞增殖及诱导分化的作用。我们还进行了反映肿瘤细胞浸润和转移能力的粘附、运动和降解侵袭实验，结果显示桂皮酸对PGCL3细胞的上述三种能力都有明显的抑制作用，表明桂皮酸在诱导PGCL3细胞向成熟方向分化的同时，还使肿瘤细胞的侵袭转移能力减弱。Highly metastatic human lung cancer cell line (PGCL3) was treated with 1-5mmol/L cinnamic acid in vitro. As a result,the PGCL3 cells had their phenotype reversed to their original normal cells in terms of morphology,proliferation rate, mitotic index, colony-forming ability in semisolid agar culture and Con A agglutination reaction. These results showed that cinnamic acid could inhibit proliferation and enhance differentiation of PGCL3 cells. We also found that cinnamic acid treatment could result in significant inhibition of adhesion of cells to laminin substrate, migration ability through the polycarbonate filter of Boyden Chamber,and invasive ability through matrigel as well. In conclusion,cinnamic acid could induce differentiation of PGCL3 cells accompanied by a decline of their invasive and metastatic abilities.

关 键 词：桂皮酸 诱导分化 侵袭能力 肺肿瘤 PGCL3 

分 类 号：R734.2[医药卫生—肿瘤] R73－3[医药卫生—临床医学]