春砂仁多糖的微波-水溶液提取及纯化工艺研究  

Optimized technology for microwave-aqua extraction and purification of Amomum villosum L.polysaccharides

作　　者：樊亚鸣[1] 罗月霞[1] 陈永亨[1] 罗亚红[1] 

机构地区：[1]广州大学化学化工学院,广州510006

出　　处：《农业工程学报》2007年第7期218-221,共4页Transactions of the Chinese Society of Agricultural Engineering

基　　金：国家星火计划项目(2005EA780029);广东省科技计划项目(2006B20901006);广州市科技计划项目(20050916)

摘　　要：为综合利用春砂仁中多种有效成分,该文设计三级提取方案,先除去杂质并回收芳香及呈味成分,然后采用微波-水溶液法提取春砂仁多糖,以前级温和的条件保护后级提取产物(多糖)的特性。通过GC-MS分析得到春砂仁低极性组分中所含的挥发性成分;然后提高提取剂的极性,通过单因素及正交试验分别选择了水溶液提取春砂仁多糖的最佳工艺条件。结果表明:液固比10∶1(V/W),微波功率600 W,提取温度80℃,时间20 min,春砂仁粗多糖产率为11.33%;纯化粗多糖的条件:Sevag试剂中氯仿与正丁醇的比例为3∶1,萃取3次,所得纯化多糖的产率为50.30%;干燥多糖的条件:温度-49～-56℃,压强24～40 kPa,干燥时间4 h。For utilizing the different effective components in Amomum villosum L. compositively, three-grade of microwave extractions were designed. The impurities in the sample were removed, while the flavor and taste ones were reclaimed at the same time, and then Amomum villosum L. polysaccharides were gained by microwave-aqua extraction. In the process, the previous gentle extraction protected the trait of the later products. The experiment procedure is that the lower polarity chemical components in Amomum villosum L. were removed by ether; followed by raising the polarity of solvents, the middle polarity ones were removed by 95% ethanol, and then polysaccharides was extracted by aqua. The single factor and orthogonal experiments were carried on to choose the best technics for extracting the polysaccharides. Results are as follows： solvent/solid ratio 10 ： 1 （V/W）; microwave power 600 W; time 20 min, and the corresponding yield of Amomum vfllosum L. polysaccharides is 11.33 % the conditions of purifying the polysaccharides： the proportion of CHCl3 and n-butanol in Sevag reagent is 3 ： 1; the time of extraction is 3; the yield of the purified polysaccharide is 50. 30% the dry conditions： temperature -49--56℃ pressure 24-40 kPa and time 4 h.

关 键 词：春砂仁 多糖 微波-水溶液提取 纯化 

分 类 号：TS201.1[轻工技术与工程—食品科学]