克劳氏芽孢杆菌(Bacillus clausii S-4)吸附Zn^(2+)的研究  

Study on adsorption of Zn^(2+) by Bacillus clausii S-4

作　　者：范瑞梅[1] 张保国[1] 张洪勋[1] 范家恒[2] 王谦[1] 白志辉[1] 

机构地区：[1]中国科学院生态环境研究中心,北京100085 [2]昆明理工大学生物与化学工程学院,昆明650024

出　　处：《环境工程学报》2007年第8期44-47,共4页Chinese Journal of Environmental Engineering

基　　金：国家重点基础研究发展计划"973"项目(2004CB719704)

摘　　要：为了验证生物吸附剂去除废水中重金属离子Zn2+的可行性,筛选了一株高效吸附Zn2+的微生物菌株克劳氏芽孢杆菌Bacillus clausiiS-4。采用火焰原子吸收、红外光谱和扫描电镜能谱分析,对这种新型生物吸附剂在水相中吸附Zn2+的性能和机理进行了研究。结果表明,B.clausiiS-4菌体在20℃、30℃、40℃条件下,吸附Zn2+的最佳pH为4.5,吸附容量为57.5 mg/g,吸附容量随温度的升高而增加,吸附过程是吸热反应,吸附平衡时间约30 min。吸附前后,B.clausiiS-4菌体表面上的化学官能团和金属离子发生了明显的变化,—OH、—NH4+、—COOH、(—CO—NH—)、—C6H5等官能团可能参与了吸附过程。0.1 mol/L HNO3、HCl和EDTA对锌的解吸附效果较好。在矿山废水中,B.clausiiS-4菌体对Zn2+的吸附效果也比较理想,显示出其潜在的应用价值。Bacillus clausii S-4 as a novel biosorbent of Zn^2＋ in aqueous solution was investigated. The optimum pH values for the biosorption at the three different temperatures 20 ℃ , 30 ℃ and 40 ℃ , were all determined as 4.5. The maximum equilibrium biosorption capacity of B. clausii S-4 was 57.5 mg/g at 40 ℃. The thermodynamic conclusion showed that the biosorption process were endothermic and spontaneous. Fourier transform infrared analysis （FTIR） indicated the possible functional groups participated during the adsorption of Zn^2＋ were--OH, --NH4, --COOH, （--CO--NH--） and --C6H5. The energy dispersive X-ray analysis （EDX） in the biomass loaded with Zn^2＋ shows the presence of zinc bands. The desorption of Zn^2＋ was efficient in 0. 1 mol/L HNO3, HC1 and EDTA solution. The adsorption of Zn^2＋ by B. clausii S-4 in mine drainage wastewater was also efficient. These results showed that B. clausii S-4 biomass have a potential capacity in wastewater treatment for Zn^2＋ removal.

关 键 词：生物吸附剂 克劳氏芽孢杆菌 吸附容量 红外光谱 X射线能谱分析 

分 类 号：Q939.99[生物学—微生物学]