星形胶质细胞培养方法的改进  

Improvement of Astrocytes Primary Cultivation

作　　者：黄柏胜[1] 刘发益[1] 曹莉[1] 贺芳[1] 赵彦[1] 邬力祥[1] 

机构地区：[1]中南大学湘雅医学院生理学系,湖南长沙410078

出　　处：《实用预防医学》2007年第4期981-983,共3页Practical Preventive Medicine

基　　金：湖南省卫生厅科研基金(B2005-003)

摘　　要：目的改进大鼠大脑皮质星形胶质细胞的体外培养方法,为研究星形胶质细胞的生物学作用提供实验模型。方法新生1～3d的大鼠,无菌操作下取双侧大脑皮质,机械吹打使细胞分散成悬液,差速粘附处理以去除成纤维细胞成分,细胞悬液原代培养9～14d,待细胞融合成单层并铺满瓶底后,4次消化传代,GFAP免疫组化染色鉴定星形胶质细胞。结果这种改进的星形胶质细胞培养方法,分离培养出星形胶质细胞,纯化率达95%以上。结论采用本实验改进的方法,培养的大鼠星形胶质细胞生长良好,纯度高,为后续实验对星形胶质细胞的生物学作用研究奠定实验基础。Objective The cultivation method for rat cerebral astrocytes in vitro was studied and modified to provide experimental models for astrocyte research. Methods Rats aged i - to 3 - day were used in the experiment, Ambi-cerebral cortex was dissected under sterile operation and cells were suspension through a mechanical method. Differential attachment was used to remove contaminating fibroblasts. The remaining cells were cultured for 9- 14 days, After cells were confluent in a monolay on the bottom of the culture flask, the cells were digested. The 4th generation cells were used and the passaged cells were identified as astrocytes by immunocytochemical staining with anti- GFAP antibody. Result The rat astrocyte was successfully isolated and the proportion of astrocyte was 〉 95 %. Conclusions This modified cultivation method of astrocyte from rat cerebral tissue is established with high purity, and in a good growth condition.

关 键 词：大脑皮质 星形胶质细胞 细胞培养 

分 类 号：R-332[医药卫生]