大鼠髁状突软骨细胞体外分离培养和压力模型的构建  

Culture of SD Rat Chondrocytes and Establishment of Mechanical Pressure Model in Vitro

作　　者：黄生高[1] 王明朗[1] 钟孝欢[1] 王会欣[1] 

机构地区：[1]中南大学湘雅二医院口腔科,湖南长沙410011

出　　处：《实用预防医学》2007年第4期1013-1017,共5页Practical Preventive Medicine

摘　　要：目的培养大鼠髁状突软骨细胞(Mandibular Condylar Chondrocytes,MCCs),建立简便易行的髁状突软骨细胞持续静压力模型。方法SD大鼠处死后无菌条件下分离髁状突软骨,利用机械—酶二次消化法分离细胞并培养传代,鉴定细胞来源,观察细胞生长情况,绘制生长曲线;在压力模型给细胞加压后,检测培养细胞的形态、增殖等。结果成功获得MCC有限细胞系,给细胞加压48h有促进细胞增殖的作用,90kpa压力抑制细胞增殖。结论利用机械—酶二次消化法,可成功培养出原代SD大鼠MCCs,自制压力模型可作为加压装置对细胞实施加压干预。Ohjective To obtain a finite cell line of chondrocYtes from SD rats, and to establish a static pressure model. Methods The mandibular chondylars were obtained by digested the cartilage with mechanical - enzyme methods from 2 - week agedrats. The origin of the cells was identified, the status of cell growth was observed, and a growth curve was probed. Cells were cultured in the pressure model and their shape and proliferation were monitored. Results A MOOs finite cell line was successfully obtained. The suitable pressure enhanced the MOOs＇ proliferation, while the overloaded pressure inhibited the MOOs＇ proliferation. Conclusions We can obtain MCCs successfully by using mechanical- enzymemethods, and the pressure model satisfies the requirements of compressive loading on cell.

关 键 词：细胞培养 软骨细胞 压力 

分 类 号：R-332[医药卫生]