实验性大鼠脑出血血肿周围组织胶质细胞纤维酸性蛋白的动态表达  

The dynamic expression of Glial Fibrillary Acidic protein in peri-cerebral Hemorrhage of rat model

作　　者：张彤[1] 刘瑞春[1] 张祥建[1] 

机构地区：[1]河北医科大学第二医院神经内科,石家庄050000

出　　处：《脑与神经疾病杂志》2007年第4期243-246,共4页Journal of Brain and Nervous Diseases

摘　　要：目的:动态观察胶质细胞纤维酸性蛋白(GFAP)在脑出血动物模型脑组织中的表达及其意义。方法应用脑立体定向技术,建立大鼠脑出血模型,假手术作为对照组。分别测定脑组织含水量,观察血肿周围GFAP的表达规律。结果:大鼠脑出血后脑水肿于48h达到高峰,与对照组比较有统计学意义(P<0.05)。脑出血后脑内GFAP阳性细胞数6h表达最强,在72h时表达最少,7d的阳性细胞数量比72h增加(P<0.05),但数量少于6h(P<0.05)。相应时间点出血组阳性细胞数与对照组比较,有明显统计学意义,(P<0.05)。结论:脑出血后脑内GFAP的表达与脑水肿相关,提示星形胶质细胞在脑出血的病理变化过程中有重要作用。星形胶质细胞在损伤的修复作用具有二重性,即可以抑制也可以促进损伤修复。Objective： The purpose of this research is to investigate the role of glial fibrillary acidic protein （GFAP） in intracerebral hemorrhage （ICH） of animals model. Methods： ICH rat model was made by stereotactic technology, and sham injections served as controls. We measured brain water content, and GFAP expression after ICH. Results： The measured results show that the brain edema achieves the peak value 48 hours after ICH. The brain edema is statistically significantly higher than that of the comparison group （P〈0.05）. The number of the masculine cells with obvious GFAP immunity reaction around haematoma area was observed to achieve the highest at 6 hours and the lowest at 72 hour after ICH. Seven days after ICH, the quantity of masculine cells increased significantly comparing to that after 72 hours （P〈 0.05） but less than the quantity after 6 hours （P〈0.05）. The ICH group of those masculine cells with the comparison group at different times demonstrates the validation of this study method statistically （P〈0.05）. Conclusion： The GFAP reactions in intracerebral hemorrhage area are highly related to brain edema, indicating that astrocyte plays a pivotal role in the pathological change of intracerebral hemorrhage. It either restrains or expedites the reparation of the intracerebral hemorrhage.

关 键 词：脑出血 胶质细胞纤维酸性蛋白 星形胶质细胞 脑水肿 大鼠 

分 类 号：R743.34[医药卫生—神经病学与精神病学]