肿瘤坏死因子α基因多态性与多发性硬化的相关性研究  

Association between tumor necrosis factor alpha gene polymorphism and multiple sclerosis

作　　者：宋秀娟[1] 刘瑞春[1] 杨静[1] 侯慧清[1] 刘静[1] 郭力[1] 

机构地区：[1]河北医科大学第二医院神经内科,050000

出　　处：《脑与神经疾病杂志》2007年第4期280-282,共3页Journal of Brain and Nervous Diseases

基　　金：河北省卫生厅技术跟踪资助项目(编号:200402)

摘　　要：目的:研究肿瘤坏死因子α(TNFα)基因多态性与多发性硬化(MS)的相关性。方法:①采用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)技术对58例MS患者和79名健康人进行TNFα基因多态性分析。②对MS组患者分别进行扩展病残状态评分(expanded disability status scale,EDSS)、首次发病年龄、病程、发病次数临床资料收集。结果:①MS组TNFα基因型分布及等位基因频率与正常对照组比较均无明显差异(χ2=0.466,P=0.495;χ2=0.229,P=0.632)。②基因型为TNFα1/1、TNFα1/2、TNFα2/2的EDSS评分、首次发病年龄、病程及发病次数各组间比较差异均无统计学意义(F=0.53,P=0.5914;F=1.34,P=0.2699;F=0.37,P=0.6914;F=0.49,P=0.6182)。结论:TNFα等位基因多态性与MS的易患性、EDSS评分、首次发病年龄、病程及发病次数均无显著相关性。Objective： To investigate the relationship of the tumor necrosis factor alpha（TNFα） gene polymorphism to multiple sclerosis （MS）. Methods： ①A case-control research was performed on 58 MS patients and 79 healthy individuals as control group. The gene polymorphism at position -308 in the promoter region of TNFα was screened by polymerase chain reaction （PCR） amplification and fragment length polymorphism （RFLP） assay. ②The neurologic impairment status of patients was scored according to the Expanded Disability Status Scale（EDSS）,meanwhile collecting clinical data-the age at onset, the duration of disease, and the number of relapses. Results.. ①No significant difference in genotype distribution and allele frequencies of TNFα existed in MS group compared with the control group （X^2=0. 466 ,P= 0. 495, X^2= 0. 229,P=0. 632,respectively）. ②There was no significant difference in EDSS scores, the age at onset, the duration of disease, and the number of relapses among MS group with TNFα1/1 , TNFα1/2 and TNFα2/2 respectively （F=0.53,P =0. 5914; F=1. 34,P=0. 2699;F=0.37,P=0. 6914;F=0.49,P=0. 6182,respectively）. Conclusions： TNFα gene polymorphism at position -308 in the promoter region might not be associated with MS genetic susceptibility. There was no significant relationship of TNFα gene polymorphisms to EDSS scores, the age at onset , the duration of disease and the number of relapses.

关 键 词：肿瘤坏死因子 多发性硬化 基因 多态性 EDSS评分 

分 类 号：R739.63[医药卫生—肿瘤]