当归补血汤挥发油的气相色谱-质谱指纹图谱研究  

GC-MS Fingerprint of Volatile Oil from Danggui Buxue Decoction

作　　者：黄水清[1] 魏刚[2] 黄月纯[2] 刘东辉[1] 

机构地区：[1]广州中医药大学,广东广州510405 [2]广州中医药大学第一附属医院,广东广州510405

出　　处：《中国实验方剂学杂志》2007年第8期1-3,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广东省科技计划项目课题(2003C33903);广东省中医药管理局课题(103025)

摘　　要：目的:对当归补血汤中挥发油进行分析,建立其挥发油的气相色谱-质谱联用(GC-MS)指纹图谱。方法:采用GC-MS方法分析挥发油主要组分。在进样口温度250℃,接口温度230℃,载气流速1.3 mL.min-1,柱压为100 kPa,升温速率3℃/min的色谱条件下,挥发油主成分可达到较好分离。结果:当归补血汤中挥发油主要有11个共有峰,其中主要成分为Z-藁本内酯、亚丁基苯酞、正丁基苯酞、E-藁本内酯等。结论:该指纹图谱可作为当归补血汤挥发油稳定性的参考。Objective：To study the main components of volatile oil from Danggui Buxue decoction, and establish the GC-MS fingerprint of its volatile oil. Methods： The main components of volatile oil were analyzed by GC-MS. The injector temperature was 250 ℃. The interface temperature was 230 ℃. The flow rate of the carrier gas was 1.3 mL·min^-1. The progrmnmed temperature rate was 3 ℃ · mln-1 . Results： 11 peaks were separated on GC-MS fingerprint. The main components were tested as trans-ligustilide, butylidene phthalide, n-butylphthalide, and cis-ligustilide. Conclusions：The fingerprint can be used as a referrence for monitoring the stability of volatile oil of Danggui Buxue decoction.

关 键 词：当归补血汤 挥发油 指纹图谱 气相色谱-质谱联用 

分 类 号：R284.1[医药卫生—中药学]