猪肺表面活性物质对大鼠早期脂多糖性急性肺损伤的治疗作用  

Porcine surfactant in treatment of LPS-induced early-stage acute lung injury in rats

作　　者：王轶群[1] 孙瑜[1] 杨瑞[1] 钟纪根[1] 卢建[1] 

机构地区：[1]第二军医大学基础部病理生理学教研室,上海200433

出　　处：《中国病理生理杂志》2007年第8期1583-1586,共4页Chinese Journal of Pathophysiology

基　　金：上海市科委重点基础资助项目(No.03JC14002);军队十一五科技攻关资助项目(No.06G63)

摘　　要：目的:观察猪肺表面活性物质(PPS)混悬液对大鼠早期脂多糖(LPS)性急性肺损伤(ALI)的治疗作用。方法:SD大鼠随机分为4组:生理盐水组、低、中、高3个不同剂量PPS给药组,各组动物均气道内滴注LPS1.5 mg.kg-1,30 min后分别经气管滴注100 mg.kg-1、150 mg.kg-1、200 mg.kg-1PPS(PPS组)或等量生理盐水(对照组)。之后观察6 h,监测大鼠的PaO2和PaCO2,计算各组大鼠存活率。大鼠处死后检测肺系数(LI),肺泡灌洗液(BALF)中总蛋白(TP)含量,白细胞(WBC)数和肿瘤坏死因子(TNF)-α的浓度,并观察肺病理组织学改变。结果:与生理盐水组相比,LPS损伤大鼠早期气道内滴入外源性PPS可提高PaO2,降低PaCO2,提高存活率,减轻肺水肿及肺毛细血管膜的通透性,PPS150组、PPS200组的治疗效果优于PPS100组。结论:PPS早期给药对LPS气道内滴注致ALI具有明显的治疗作用。AIM： To evaluate the therapeutic efficacy of intratracheal instillation of porcine pulmonary sudacrant （PPS） in rats with lipepelysaccharides （LPS） - induced early - stage ALl in this study. METHODS ： SD rats weighing 200 g-300 g were randomly divided into 4 groups： LPS （1.5 ·kg^-1 ） ＋saline, LPS ＋PPS 100 ·kg^-1 , LPS ＋ PPS 150 ·kg^-1 , LPS ＋ PPS 200 ·kg^-1. The PaO2 and PaCO2, as well as survival rate of rats were examined for 6 h after the start of PPS - instillation. Then, rats were killed and lungs were immediately removed for lung index （ LI ） and histological analysis. The bronchoalveolar lavage fluid （BALF） was collected for measurement of total protein （TP） contents, TNF-α level and white blood cell（WBC） numbers. RESULTS： Significantly increased PaO2, reduced mortality rate, decreased total protein and TNF -α contents in BAL, as well as lung index and meliorated histological appearance were observed in three PPS - treated groups compared with group given saline after LPS （ P 〈 0. 05 ）. The therapeutic effect in PPS150 and PPS200 groups was better than that in PPS100 group. CONCLUSION： Intratracheal PPS instillation provides protective effect on acute lung injury in rats induced by LPS.

关 键 词：急性肺损伤 脂多糖类 猪肺表面活性剂 大鼠 

分 类 号：R363[医药卫生—病理学]