浸提法测定玉米叶绿素含量的改进  

Improved Extraction Methods of Chlorophyll from Maize

作　　者：明华[1] 胡春胜[1] 张玉铭[1] 程一松[1] 

机构地区：[1]中国科学院遗传与发育生物学研究所农业资源研究中心

出　　处：《玉米科学》2007年第4期93-95,99,共4页Journal of Maize Sciences

基　　金：中国科学院知识创新项目(KSCXZ-YW-N-037)

摘　　要：以玉米叶片为试材,研究了8种不同有机溶剂浸提液提取叶绿素的效率及低温冷冻对叶绿素提取的影响。试验中采用叶绿素仪先预叶片;冷冻—高温快速浸提试验,采用先称取样品,后冷冻方式。结果表明:室温(25℃)下,浸提法要优于研磨法,混合液浸提叶绿素比单独用丙酮或乙醇浸提快,其中丙酮∶乙醇=2∶1的浸提液浸提速度快而稳,是试验中最佳的叶绿素提取方法。最佳浸提时间在浸提16h时。叶片冷冻处理后,快速浸提能够缩短叶绿素浸提时间。由于受光照、高温的影响,测定值普遍较低。The extraction efficiencies of chlorophyll from maize leaves after frozen treatment were co eight determined methods using different organic solvents. We used leaves before freezing. The result showed that soaking mpared on SPAD-502 before sampling, weighed up the methods were better than Arnon method at 25 ~C. It was better to measure the efficiencies of chlorophyll using mixture solution than using acetone or ethanol lonely. We got the collusion that methods of acetone mixed with ethanol （2：1） was the best of all, and the best time at 16h after soaking. The methods of frozen treatment before soaking could advance the speed of chlorophyll, but the number decreased because of the light or high temperature.

关 键 词：玉米 叶绿素 浸提 有机溶剂 

分 类 号：S513[农业科学—作物学]