ELISA法检测赭曲霉毒素A的改进研究  

Research on Determination of Ochratoxin A by Improved ELISA Method

作　　者：时瑾[1] 黄飚[2] 孙蔚榕[1] 张珏[2] 赵晓联[1] 

机构地区：[1]江苏省微生物研究所有限责任公司,江苏无锡214063 [2]江苏省原子医学研究所,江苏无锡214063

出　　处：《食品科学》2007年第8期425-428,共4页Food Science

摘　　要：针对ELISA法检测赭曲霉毒素A(OTA),使用微振荡酶标板可以缩短免疫反应检测时间约30min。采用ELISA方法检测小麦样品中的OTA时,使用空白样品提取液来配制OTA标准溶液可以消除样品基质造成的干扰。同时对提取溶剂加以改进,采用NaCl-水-甲醇(10%:70:30)作为提取溶剂的提取效率最高。0.1、1、10μg/kg三个浓度水平的加标回收率为95%～110%,方法的重现性很好,变异系数小于10%。Tiny-shake was used to shorten the detection time of enzyme linked immunosorbent assay （ELISA） for ochratoxin A （OTA）. OTA from wheat was determined by ELISA. It was validated that preparing the standard solution of OTA with wheat extract solution could eliminate interference of substances in wheat. The extraction solvent was also optimized. Results showed that higher extraction rote can be achieved with NaC1-H20-methanol （10%：70：30）. The recoveries of OTA spiked at three levels, namely, 0.1, 1, 10 v g/kg were 102%, and the coefficient of variation was less than 10%.

关 键 词：赭曲霉毒素A:ELISA 提取溶剂 小麦 

分 类 号：TS210.7[轻工技术与工程—粮食、油脂及植物蛋白工程]