电刺激小脑顶核对成年大鼠局灶脑缺血/再灌注后脑内Nestin表达的影响  

Effect of Cerebellar Fastigial Nucleus Stimulation on the Expression of Nestin in Adult Rat Brain after Focal Cerebral Ischemia/Reperfusion

作　　者：黄艳君[1,2,3] 罗勇[1,2,3] 

机构地区：[1]重庆医科大学附属第一医院神经内科 [2]重庆市神经病学重点实验室,重庆市400016 [3]绵阳市第三人民医院神经内科,四川绵阳市621000

出　　处：《中国康复理论与实践》2007年第8期706-709,共4页Chinese Journal of Rehabilitation Theory and Practice

基　　金：重庆市科委攻关项目资助(渝科发计字[2003]43号文;2003-7);重庆市卫生局科研项目资助(渝卫科教[2005]31号文;编号:2005-2-179)

摘　　要：目的探讨电刺激小脑顶核(FNS)对成年大鼠局灶脑缺血/再灌注后脑内不同部位、不同时间点Nestin表达的影响。方法采用线栓法制备大鼠右侧大脑中动脉局灶脑缺血/再灌注模型。180只雄性Wistar大鼠随机分为正常对照组(NC组)、假手术组(SC组)、局灶脑缺血/再灌注组(I/R组)、局灶脑缺血/再灌注+假FNS组(I/RFs组)、局灶脑缺血/再灌注+FNS组(I/RF组),每组36只,并根据再灌注时间的不同又分为1d、3d、7d、14d、21d和28d6个亚组(n=6)。缺血时间均为1h,于再灌注后立即刺激对侧小脑顶核1h。应用免疫组织化学技术检测缺血侧侧脑室、海马Nestin阳性细胞的动态表达。结果局灶脑缺血/再灌注后不同时间点Nestin阳性细胞在缺血侧侧脑室区、海马齿状回的表达均有增加,呈单峰变化趋势,7d达高峰(P<0.01),14d后逐步下降(P<0.01),而FNS后,Nestin阳性细胞数量增加更加明显(P<0.05,P<0.01),7d达到高峰(P<0.01),14d后仍维持在较高水平(P<0.01),且Nestin阳性细胞形态也发生明显变化。结论FNS可促进局灶脑缺血/再灌注后脑内Nestin阳性细胞数量增加。Objective To explore the effect of cerebellar fastigial nucleus stimulation （FNS） on the expression of Nestin in adult Wistar rat brain after focal cerebral ischemia/reperfusion. Methods The animal model of focal cerebral ischemia/reperfusion was made by filament occlusion of the right middle cerebral artery. 180 male Wistar rats were randomly divided into five groups： normal control group （NC group）, sham operation control group （SC group） , ischemia/reperfusion group （I/R group）, ischemia/reperfusion treated with sham FNS group （I/RFs group）, and ischemia/reperfusion treated with FNS group （I/RF group）, each group contain 1 d, 3 d, 7 d, 14 d, 21 d and 28 d six time points （for each point, n=6）. Immunohistochemistry method was used to detect the number of Nestin expression positive cells following various time and interference in lateral cerebral ventriculus and hippocampus in adult Wistar rat brain. Results After focal cerebral ischemia/reperfusion, the number of Nestin positive cells increased at each time point, reached small peak value in 7th d （P 〈0.01）. After treated with FNS, the number of Nestin positive cells increased more strikingly at each time point （ P〈0.05, P 〈0.01）, reached higher peak value in 7th d （ P〈0.01） , and maintained at higher level in 14th d. Furthermore, the shape of Nestin positive cells changed significantly. Conclusion FNS can increase the number of Nestin positive cells in some brain regions after focal cerebral ischemia/reperfusion.

关 键 词：小脑顶核刺激 局灶脑缺血/再灌注 NESTIN 大鼠 

分 类 号：R743.3[医药卫生—神经病学与精神病学]