活动性狼疮肾炎患者外周血单个核细胞钙调神经磷酸酶活性的检测及白细胞介素-4表达变化  

Significance of Calcineurin Activation and Interleukin-4 Expression in Patients with Active Lupus Nephritis

作　　者：梁鸣[1] 阳晓[2] 李幼姬[2] 秦曙光[1] 傅君舟[1] 

机构地区：[1]广东省广州市第一人民医院肾内科,广州510180 [2]中山大学附属第一医院,广州510080

出　　处：《中国中西医结合肾病杂志》2007年第8期440-443,共4页Chinese Journal of Integrated Traditional and Western Nephrology

基　　金：广东省自然科学基金博士科研启动项目(No.5300319)

摘　　要：目的:检测活动性狼疮肾炎(LN)患者外周血单个核细胞(PBMC)白细胞介素-4(IL-4)表达及其与钙调神经磷酸酶(Calcineurin,CaN)活性的关系。方法:体外培养活动性LN患者PBMC,应用发色底物法检测胞浆CaN活性,逆转录PCR检测IL-4 mRNA表达。结果:(1)在单纯培养情况下,正常对照组和LN组PBMC均出现一定量CaN活化,活动性LN组显著高于正常对照组[(46.08±5.58)mmol/mg.pr vs(8.81±3.61)mmol/mg.pr,P<0.01];在PMA+Ionomycin刺激下,各组CaN活性均升高,活动性LN组CaN活性明显高于正常对照组[(69.34±12.59)mmol/mg.pr vs(37.12±11.57)mmol/mg.pr,P<0.01];(2)LN患者PBMC在单纯培养和PMA+Ionomycin刺激时IL-4蛋白和mRNA表达均显著高于相应的对照组(P<0.05);(3)在单纯培养和PMA+Ionomycin刺激时,FK506对LN PBMC表达IL-4蛋白和mRNA均有显著抑制作用(P<0.01)。结论:LN患者PBMC存在CaN过度活化;LN患者PBMC高效表达IL-4与其CaN过度活化密切相关,通过阻断CaN活性可调控IL-4表达。Objective： To investigate the significance of the calcineurin （CAN） activation in active lupus nephritis patients. Methods：Peripheral blood mononuclear cells（PBMCs） were separated from twenty- one active LN patients and 12 healthy controls. Phphatase activity of CaN was determined by using the CaN assay kit to measure the amount of released PO 4. Reverse transcription polymerase chain reaction （RT- PCR） was used to detect the expression of IL- 4 mRNA. Results： （1）Increased activation of CaN in spontaneous cultured PBMC in active LN group was found, compared with control group （46.08 ±5.58 vs 8.81 ±3.61, /9〈0.01）. Stimulated by PMA/Ionomycin, CaN activity in active LN group was also higher than that of control（69.34 ±12.59 vs 37.12 ±11.57, P 〈 0.01 ） ; （2）Relative amounts of IL- 4 in PBMC in active LN groups increased significantly as compared with the control groups under spontaneous and PMA/lonomycin - induced culture, respectively （P 〈 0.01 ） ; （3） FK506 reduced significantly production of IL-4 in spontaneous and PMA/Ionomycin- induced PBMC of LN. Conclusion： These results indicated that elevated activation of CaN in active LN might participate in regulation overexpression of IL- 4 in PBMC of LN. Through inhibiting CaN activity, FK506 may prevent abnormal activation of Th2 cell in lupus nephritis.

关 键 词：狼疮肾炎 外周血单个核细胞 钙调神经磷酸酶 INTERLEUKIN-4 白细胞介素-4 

分 类 号：R593.242[医药卫生—内科学]