消痞颗粒对大鼠慢性萎缩性胃炎伴不典型增生胃黏膜Bcl-2和Bax表达的影响  

Influence of Xiaopi Granules on the expression of Bax & Bcl-2 in gastric mucosa of the rats with chronic atrophic gastritis accompanied with dyspalsia

作　　者：孟捷[1] 杨晋翔[1] 鲁香凤[2] 戴欣[2] 方杰[2] 

机构地区：[1]北京中医药大学东方医院消化科,北京100078 [2]北京中医药大学东直门医院病理科,北京100700

出　　处：《中国中西医结合消化杂志》2007年第4期214-217,共4页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

基　　金：教育部科学技术研究重点项目(01031)

摘　　要：[目的]从胃黏膜Bcl-2和Bax表达变化的角度探讨实验性慢性萎缩性胃炎(CAG)伴不典型增生(A)发病机制及消痞颗粒对其治疗作用的机制。[方法]将大鼠随机分为5组,采用综合法(幽门弹簧插入加高盐热糊法)复制大鼠CAG伴ATP模型,免疫组化法显示胃黏膜Bcl-2和Bax表达。[结果]正常大鼠胃黏膜基底有Bcl-2阳性表达,在胃黏膜ATP区域Bcl-2阳性表达增多(P<0.01),应用消痞颗粒剂治疗后,胃黏膜Bcl-2阳性表达明显减弱,与模型组、自然恢复组相比差异有统计意义(P<0.01);正常大鼠胃窦部黏膜的增殖带有Bax蛋白表达,而模型组Bax阳性表达显著减弱,经消痞颗粒治疗Bax表达有一定增强。[结论]消痞颗粒治疗后胃黏膜病变病理形态明显改善,可能是其通过抑制Bcl-2表达、上调Bax表达、调节细胞增殖凋亡而起作用。[Objective]To explore the pathogenesis of experimental chronic atrophic gastritis with dysplasia and treatment mechanisms of Xiaopi granules from the view point of the changes of the expression of Bax and Bcl-2 in gastric mucosa. [Methods]The Wistar rats were randomly divided into 5 groups. The CAG model was made by using a synthetic method,a combined application of insertion of a metallic spring into the gastric pylorus, oral administration of hot salty paste （60℃-70℃, 10%NaCl）twice a week. The expression of Bax and Bcl-2 of the gastric mucous in the rats were displayed by immunohistological techniques. [Results] The positive expression of Bcl-2 in gastric mucosa of the rats in model group was significantly lower than that in normal group, and increased after treated with Xiaopi granule. The positive expression of Bax in gastric mucosa of the rats in model group was significantly higher than that in normal group, whereas the positive expression of Bax was decreased after treated with Xiaopi granule. [Conclusion]Inhibiting the positive expression of Bcl-2 and strengthening the positive expression of Bax may be one of the mechanisms Of Xiaopi granule to treat CAG and dysplasia.

关 键 词：胃炎 萎缩性 消痞颗粒 不典型增生 增殖凋亡 

分 类 号：R573.3[医药卫生—消化系统]