芩连合剂对实验性胃溃疡大鼠胃黏膜细胞内钙及氧自由基的影响  

Effects of Qinlianheji on the Ca^(2+) and oxygen free radical of gastric mucous cell in rats with acute gastric ulcer

作　　者：张俊杰[1] 林庚庭[2] 周庚生[1] 吴明清[1] 

机构地区：[1]浙江中医药大学基础医学院中医基础理论教研室,浙江杭州310053 [2]解放军第117医院门诊部,浙江杭州310004

出　　处：《中国中西医结合消化杂志》2007年第4期218-221,共4页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

基　　金：浙江省中医药管理局资助项目(2003Y003)

摘　　要：[目的]探讨芩连合剂对胃溃疡(GU)的保护作用及其作用机制。[方法]采用无水乙醇灌胃法复制大鼠急性GU模型,测定胃黏膜细胞内丙二醛(MDA)、超氧化物歧化酶(SOD)水平、谷胱甘肽过氧化物酶(GSH-Px)活力及钙离子(Ca2+)浓度。[结果]模型组大鼠胃黏膜细胞内Ca2+浓度及MDA表达较其他各组明显升高,SOD、GSH-Px的表达明显降低,差异均有统计学意义(均P<0.01)。[结论]芩连合剂通过降低胃黏膜细胞内Ca2+浓度及MDA的表达,提高SOD和GSH-Px的表达,起到保护胃黏膜的作用,从而发挥其抗GU的作用。[Objective]To study the effect and mechanism of Qinlianheji（QLHJ） on protecting gastric mucous membrane of exprimental gastric ulcer in rats. [Methods]The acute gastric mucosa damage was induced by dehydrated alcohol in rats, the contents of SOD and MDA , the activity of GSH-Px and the concentration of Ca^2＋ was detected in the gastric mucous membrane cell. [Results] Intracellular SOD and GSH-Px in the model group was significantly lower and the concentration of Ca^2＋ and MDA was significantly higher than those in other control group. And the difference was significant（P〈0. 01） . [Conclusion]QLHJ can protect the gastric mucous membrane by decreasing the potency of Ca^2＋ and the contents of MDA of the gastric mucous membrane cell and rising the contents of intracellular SOD and GSH-Px, which may be one of the mechanisms of the antiulcer effect of QLHJ.

关 键 词：胃溃疡 急性 芩连合剂 大鼠 氧自由基 细胞内CA^2+ 

分 类 号：R573.1[医药卫生—消化系统]